Insights into Metatranscriptome, and CAZymes of Buffalo Rumen Supplemented with Blend of Essential Oils
- PDF / 1,040,248 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 110 Downloads / 175 Views
ORIGINAL RESEARCH ARTICLE
Insights into Metatranscriptome, and CAZymes of Buffalo Rumen Supplemented with Blend of Essential Oils Anju Kala1 • D. N. Kamra1 • N. Agarwal1 • L. C. Chaudhary1 • C. G. Joshi2
Received: 31 January 2020 / Accepted: 2 June 2020 Ó Association of Microbiologists of India 2020
Abstract The aim of this study was to investigate the rumen microbial diversity and functionality in buffaloes fed with a blend of essential oils (BEO) using LSD switch over design. The BEO consisting of blend of Trachyspermum copticum (Ajwain) oil, Cymbopogon citratus (lemon grass) oil and Syzygium aromaticum (clove bud) oleoresin mixed in equal proportion, was fed at the rate of 0, 0.75 and 1.5 ml/100 kg of body weight in 0 (control), 0.75 and 1.5 groups, respectively. The metatranscriptomic libraries of the rumen microbiome were represented by 7 domains, 84 phyla, 64 archeal genera and 663 bacterial genera with Bacteroidetes and Firmicutes constituting 80% of phyla abundance irrespective of feeding regime. Methanogenic archaea was represented by 22 phyla with Methanobrevibacter as the major genus. BEO feeding reduced the abundance of Methanococcus and Thermoplasma (P \ 0.05) at all levels. The results revealed that the feeding of BEO shifted the archeal and bacterial population at very low magnitude. The study explored the vast diversity of buffalo rumen bacteria and archaea, and the diverse wealth of rumen enzymes (CAZymes), which revealed that a major part of CAZymes comes from the less known rumen microbes indicating alternative paths of fiber degradation along with the very well known ones.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12088-020-00894-3) contains supplementary material, which is available to authorized users. & Anju Kala [email protected] 1
Animal Nutrition Division, Indian Veterinary Research Institute, Izatnagar, UP 243122, India
2
Department of Biotechnology, College of Veterinary Science, Anand Agricultural University, Anand, Gujarat, India
Keywords Buffalo CAZymes Essential oil Metatranscriptomics Rumen microbes
Introduction The emission of greenhouse gasses (GHGs) in the atmosphere is a serious issue and needs to be addressed urgently. Methane, one of the major GHG emitted by the livestock, not only pollutes the environment but also affects feed utilization by the animals. Essential oils (EOs) are among the major phytochemicals, well known for their antimicrobial activity. Use of EOs as rumen modifiers to control enteric methane production in ruminants is well documented [1–3]; however, variable results have been obtained in these studies. Blend of EOs should be more successful in mitigating methane production without compromising feed digestibility by virtue of synergism due to different modes of action of different EOs. Since methane inhibition and feed digestibility go hand in hand, therefore, the understanding between methanogenic archaea and fibrolytic bacteria, their interactions and their response towards anti-
Data Loading...
