Integrating transcriptomic and metabolomic analysis of hormone pathways in Acer rubrum during developmental leaf senesce
- PDF / 4,055,855 Bytes
- 22 Pages / 595.276 x 790.866 pts Page_size
- 71 Downloads / 209 Views
RESEARCH ARTICLE
Open Access
Integrating transcriptomic and metabolomic analysis of hormone pathways in Acer rubrum during developmental leaf senescence Chen Zhu1, Lu Xiaoyu2, Gao Junlan1, Xuan Yun1 and Ren Jie1*
Abstract Background: To fully elucidate the roles and mechanisms of plant hormones in leaf senescence, we adopted an integrated analysis of both non-senescing and senescing leaves from red maple with transcriptome and metabolome data. Results: Transcription and metabolite profiles were generated through a combination of deep sequencing, thirdgeneration sequencing data analysis, and ultrahigh-performance liquid chromatograph Q extractive mass spectrometry (UHPLC-QE-MS), respectively. We investigated the accumulation of compounds and the expression of biosynthesis and signaling genes for eight hormones. The results revealed that ethylene and abscisic acid concentrations increased during the leaf senescence process, while the contents of cytokinin, auxin, jasmonic acid, and salicylic acid continued to decrease. Correlation tests between the hormone content and transcriptional changes were analyzed, and in six pathways, genes closely linked with leaf senescence were identified. Conclusions: These results will enrich our understanding of the mechanisms of plant hormones that regulate leaf senescence in red maple, while establishing a foundation for the genetic modification of Acer in the future. Keywords: Acer rubrum, Transcriptome, Metabolomics, Hormone pathways, Leaf senescence
Background The senescence of plant leaves is a proactive and genetically precise process that is regulated by a series of internal and external factors [1]. Many environmental stresses, such as extreme temperatures, drought, nutrient deficiencies, inadequate light/shadowing, or complete darkness, and biotic stress (e.g., bacterial infections) can induce senescence [2, 3]. Phytohormone levels are determined by plant age and stress during the induction and propagation of leaf senescence [4]. * Correspondence: [email protected] 1 Institute of Agricultural Engineering, Anhui Academy of Agricultural Sciences, 40 Nongkenanlu, Hefei, Anhui 230031, P.R. China Full list of author information is available at the end of the article
These internal and external factors may act alone or in combination. Among these, plant hormones are one class of organics that are generated in certain parts of plants, which can regulate almost all aspects of plant growth and development, while inducing physiological reactions at low concentrations [5]. Currently, research on the regulation and mechanisms of plant hormones in plant senescence has made clear progress [6]. According to the role of plant senescence, previous research has shown that, in general, phytohormones can be divided into two types: senescence promoters and retardants [7]. The former encompass ethylene (ET), abscisic acid (ABA), jasmonic acid (JA), salicylic
© The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which perm
Data Loading...
