Interleukin-8 Receptors CXCR1 and CXCR2 Are Not Expressed by Endothelial Colony-forming Cells
- PDF / 3,921,324 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 89 Downloads / 158 Views
Interleukin-8 Receptors CXCR1 and CXCR2 Are Not Expressed by Endothelial Colony-forming Cells Adeline Blandinières 1,2 & Xuechong Hong 3,4 & Aurélien Philippe 1,2 & Ivan Bièche 5 & Sophie Vacher 5 & Elisa Rossi 1 & Grégoire Detriche 1 & Nicolas Gendron 1,2 & Pascale Gaussem 1,6 & Coralie L. Guerin 1,7 & Juan M. Melero-Martin 3,4,8 & David M. Smadja 1,2 Accepted: 8 November 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Endothelial colony-forming cells (ECFCs) are human vasculogenic cells described as potential cell therapy product and good candidates for being a vascular liquid biopsy. Since interleukin-8 (IL-8) is a main actor in senescence, its ability to interact with ECFCs has been explored. However, expression of CXCR1 and CXCR2, the two cellular receptors for IL-8, by ECFCs remain controversial as several teams published contradictory reports. Using complementary technical approaches, we have investigated the presence of these receptors on ECFCs isolated from cord blood. First, CXCR1 and CXCR2 were not detected on several clones of cord blood- endothelial colony-forming cell using different antibodies available, in contrast to well-known positive cells. We then compared the RT-PCR primers used in different papers to search for the presence of CXCR1 and CXCR2 mRNA and found that several primer pairs used could lead to non-specific DNA amplification. Last, we confirmed those results by RNA sequencing. CXCR1 and CXCR2 were not detected in ECFCs in contrary to human-induced pluripotent stem cell-derived endothelial cells (h-iECs). In conclusion, using three different approaches, we confirmed that CXCR1 and CXCR2 were not expressed at mRNA or protein level by ECFCs. Thus, IL-8 secretion by ECFCs, its effects in angiogenesis and their involvement in senescent process need to be reanalyzed according to this absence of CXCR-1 and − 2 in ECFCs.
Keywords ECFCs . interleukin-8 . CXCR1 . CXCR2 . chemokines . RNAseq
Introduction
* David M. Smadja [email protected] 1
Innovative Therapies in Haemostasis, INSERM, Université de Paris, F-75006 Paris, France
2
Service d’Hématologie et Laboratoire de Recherches Biochirugicales (Fondation Carpentier), AH-HP, Georges Pompidou European Hospital, F-75015 Paris, France
3
Department of Cardiac Surgery, Boston Children’s Hospital, Boston, MA, USA
4
Department of Surgery, Harvard Medical School, Boston, MA, USA
5
Department of Genetics, Université de Paris and Pharmacogenomics Unit, Institut Curie, Paris, France
6
Service d’Hématologie, AH-HP, Georges Pompidou European Hospital, F-75015 Paris, France
7
Plateforme de cytométrie, Institut Curie, F-75006 Paris, France
8
Harvard Stem Cell Institute, Cambridge, MA, USA
Endothelial colony-forming cells (ECFCs) are postnatal vasculogenic cells isolated in culture and committed to the endothelial lineage [1]. On the last two decades, the interest for these cells has been growing either as a putative cell therapy product for ischemic diseases and more recently, as a liquid biopsy to expl
Data Loading...
