Internalization of the TAT-PBX1 fusion protein significantly enhances the proliferation of human hair follicle-derived m
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ORIGINAL RESEARCH PAPER
Internalization of the TAT-PBX1 fusion protein significantly enhances the proliferation of human hair follicle-derived mesenchymal stem cells and delays their senescence Bo Wang . Feilin Liu . Zinan Liu . Xing Han . Aobo Lian . Yuying Zhang . Kuiyang Zuo . Yuan Wang . Mingsheng Liu . Fei Zou . Yixu Jiang . Minghua Jin . Xiaomei Liu . Jinyu Liu
Received: 19 December 2019 / Accepted: 7 May 2020 Ó Springer Nature B.V. 2020
Abstract Objectives To express a TAT-PBX1 fusion protein using a prokaryotic expression system and to explore potential effects of TAT-PBX1 in the proliferation and senescence of human hair follicle-derived mesenchymal stem cells.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10529-020-02909-x) contains supplementary material, which is available to authorized users. B. Wang Z. Liu X. Han A. Lian Y. Zhang K. Zuo Y. Wang M. Liu Y. Jiang M. Jin X. Liu (&) J. Liu (&) Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Avenue, Changchun 130021, China e-mail: [email protected] J. Liu e-mail: [email protected] B. Wang e-mail: [email protected] F. Liu Department of Ophthalmology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130021, China F. Zou Department of Pediatrics, The First Hospital of Jilin University, 71 Xinmin Avenue, Changchun 130021, China
Results The TAT-PBX1 fusion was produced in inclusion bodies and heterogenously expressed in Rosetta (DE3) cells. Immunofluorescence staining showed that TAT-PBX1 fusion proteins were internalized by human hair follicle-derived mesenchymal stem cells. The growth rate of cells was increased after treatment with more than 5.0 lg/mL of TAT-PBX1. The rate of senescence-associated b-galactosidase positive cells was reduced in the 10.0 lg/mL TATPBX1 group (28%) than the 0 lg/mL control group (60%). Cells treated with the TAT-PBX1 fusion protein showed higher expression of p-AKT (1.22fold that of the control), which indicates that TATPBX1 activated AKT pathway after cellular uptake. Conclusions The TAT-PBX1 fusion protein increased the proliferation of hair follicle mesenchymal stem cells and delayed their senescence by activating the AKT pathway following internalization by cells. Keywords AKT Hair follicle mesenchymal stem cells PBX1 Protein purification Senescence TAT
Introduction Human hair follicle mesenchymal stem cells (hHFMSCs) are a promising candidate for tissue engineering and regenerative medicine because of their roles in
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Biotechnol Lett
hair follicle generation, skin repair, angiogenesis, and neuronal regeneration. Additional advantages of these cells include ease of isolation, low immunogenicity, and limited ethical issues. Nonetheless, application of hHF-MSCs is restricted because they show replicative senescence in long-term in vitro culture (Lu et al. 2019). Correspondingly, cellular senescence reduces the therapeutic potential
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