Intestinal mucosal bacterial diversity of antibiotic-associated diarrhea (AAD) mice treated with Debaryomyces hansenii a
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ORIGINAL ARTICLE
Intestinal mucosal bacterial diversity of antibiotic‑associated diarrhea (AAD) mice treated with Debaryomyces hansenii and Qiweibaizhu powder Haoqing Shao1 · Chenyang Zhang1 · Chunhui Wang2 · Zhoujin Tan1 Received: 20 June 2020 / Accepted: 6 August 2020 © King Abdulaziz City for Science and Technology 2020
Abstract The aim was to investigate the combined effect of Debaryomyces hansenii and Qiweibaizhu powder (QWBZP) on the bacterial diversity of the intestinal mucosa of antibiotic-associated diarrhea (AAD) mice, for the potential treatment of diarrhea, especially which is induced by administration of antibiotics. Eighteen (18) mice were randomly assigned to three equal groups of six mice, namely Normal (mn group), Placebo control (mm group) and D. hansenii and QWBZP (DQ) treatment (mdq group). Mice were gavaged with a solution (23.33 mL·kg−1·day−1) consisting of gentamicin and cefradine to establish AAD. The DQ treatment group was gavaged with DQ for 4 days, and sterile water was used as a placebo control. The metagenome DNA of the intestinal mucosal microbiota was extracted, and the 16S rRNA gene was sequenced. Analysis showed that there were 288 OTUs for the normal group, 443 for the placebo control group, and 229 for the DQ treatment group. Phylogenetically, the gut microbiota of the DQ treatment group and the normal group were closer to each other than to the placebo control group. Both the DQ and placebo-treated groups included Stenotrophomonas, Robinsoniella, Bacteroidales S24-7 group norank, Citrobacter, and Glutamicibacter, but their abundances were significantly higher in the DQ treatment group than in the placebo control group. This suggested that the combined use of D. hansenii and QWBZP overcame the influence of dysbacteriosis and could lead to the recovery of intestinal mucosal microbiota homeostasis. This positive effect is likely related to short-chain fatty acid (SCFA)-producing bacteria, such as members of Micrococcaceae, Lachnospiraceae, and Bacteroidales S24-7 group, which could play beneficial roles in protecting the mucosal barrier and stimulating the immune response in mice. Keywords Debaryomyces hansenii · Qiweibaizhu powder · Intestinal mucosa · SCFA-producing bacteria · Antibioticassociated diarrhea
Introduction The intestinal micro-ecosystem has been recognized as the largest and the most important micro-ecosystem in the organism, and the normal gut microbiota as its core component plays a crucial role in the homeostasis of the * Chunhui Wang [email protected] * Zhoujin Tan [email protected] 1
Hunan University of Chinese Medicine, Changsha, Hunan, China
Hunan Edible Fungus Research Institute, Changsha, Hunan, China
2
gastrointestinal tract (Coyte and Rakoff-Nahoum 2019; Lozupone et al. 2012; Lynch and Pedersen 2016). As such, disturbances in the normal gut microbiota can lead to a variety of pathogenic states. The first step in understanding the symbiotic relationship between gut microbiota and its host is to characterize the baseline health microbiota and the
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