Intracellular biosynthesis of PbS quantum dots using Pseudomonas aeruginosa ATCC 27853: evaluation of antibacterial effe
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(2020) 36:147
ORIGINAL PAPER
Intracellular biosynthesis of PbS quantum dots using Pseudomonas aeruginosa ATCC 27853: evaluation of antibacterial effects and DNA cleavage activities Necip Öcal1,3 · Ahmet Ceylan2 · Fatih Duman1 Received: 30 March 2020 / Accepted: 13 August 2020 © Springer Nature B.V. 2020
Abstract Bacterial biosynthesis of quantum dots (QDs) offers a green alternative for the production of nanomaterials with superior properties, such as tunable size dependent emission spectra and a long fluorescence lifetime. In this study, we have achieved intracellular production of PbS QDs using Pseudomonas aeruginosa ATCC 27853. The characterization of these PbS QDS was performed by different techniques, such as Ultraviolet–visible (UV–Vis) spectroscopy, photoluminescence (PL), X-ray diffraction (XRD), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX) and particle size analysis (DLS). The obtained results confirmed the synthesis of PbS QDs. The PbS QDs showed absorption peaks at 1088 nm (ultraviolet–visible spectrometry) and a luminescence peak at 1572 nm. In addition, the intracellular biosynthesized PbS QDs showed a particle size in the range 3.47–11.45 nm, which is consistent with DLS and sphere-shaped nanocrystals with good crystallinity and a cubic cristalline structure including pure Pb and S elements. Biosynthesized PbS QDs showed antibacterial activity against Proteus mirabilis ATCC 25933 and Escherichia coli ATCC 25922 from Gram (−) bacteria and Bacillus cereus NRLL B-3008 and Micrococcus luteus ATCC 10240 from Gram (+) bacteria. Besides, these current results obtained from the cleavage studies revealed that PbS QDs do not show DNA cleavage activity. Consequently, the microorganism-based intracellular method allows an economic and environmentally friendly way to obtain PbS QDs with superior optical properties and they have a potential to be used in healthcare applications. Keywords Quantum dots · PbS · Biosynthesis · Antimicrobial activity · DNA cleavage
Introduction The application areas of QDs have widened together with the advancement of nanotechnology. QDs have attracted great interest from material scientists to researchers working in the field, such as material and bioscience. QDs are fluorescent semiconductor nanomaterials, which occur as materials from the elements in the periodic groups of II–VI, III–V or IV–VI (e.g., ZnS, CdS, ZnTe, CdTe, InP) (McHugh et al. 2018; * Necip Öcal [email protected] 1
Faculty of Science, Department of Biology, Erciyes University, 38280 Kayseri, Turkey
2
Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Erciyes University, 38280 Kayseri, Turkey
3
ERNAM - Erciyes University Nanotechnology Application and Research Center, 38280 Kayseri, Turkey
Jadhav et al. 2017; Mal et al. 2016). They have been shown to contain about 200–10,000 atoms (Rizvi et al. 2010). Due to the effect of quantum confinement, QDs are extremely stable with wide absorption and restricted emission spectra. The emission colour of QD
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