Intracerebroventricular streptozotocin administration impairs mitochondrial calcium homeostasis and bioenergetics in mem
- PDF / 2,339,029 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 82 Downloads / 171 Views
RESEARCH ARTICLE
Intracerebroventricular streptozotocin administration impairs mitochondrial calcium homeostasis and bioenergetics in memory‑sensitive rat brain regions Yati Sharma1 · Debapriya Garabadu1 Received: 26 April 2020 / Accepted: 23 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Alzheimer’s disease (AD) is a progressive neurodegenerative disorder with cardinal manifestation of cognitive dysfunction. The limitation to avail a successful drug candidate encourages researchers to establish an appropriate animal model in the novel anti-AD drug discovery process. In this context, the mechanism of mitochondrial dysfunction in cognitive deficit animals is yet to be established for intracerebroventricular injection of streptozotocin (ICV-STZ). Experimental dementia was induced in male rats by ICV-STZ on day-1 (D-1) of the experimental protocol at a sub-diabetogenic dose (3 mg/kg) twice at an interval of 48 h into both rat lateral ventricles. ICV-STZ caused cognitive decline in terms of increase in the escape latency on D-14 to D-17 and, decrease in the time spent and percentage of distance travelled in the target quadrant during Morris water maze and decrease in the spontaneous alteration behavior during Y-maze tests in rats. Further, ICV-STZ decreased the level of acetylcholine and activity of choline acetyltransferase and increased the activity of acetylcholinesterase in rat hippocampus, pre-frontal cortex and amygdala. Interestingly, ICV-STZ increased the mitochondrial calcium in addition to decrease in the mitochondrial function, integrity and bioenergetics in all rat brain regions. Further, ICV-STZ enhanced the levels of expression of NR1 subunit of N-methyl-d-aspartate receptor, mitochondrial calcium uniporter and sodium-calcium exchanger in these rat brain regions. Thus, NR1-dependent mitochondrial calcium accumulation could be considered as a major attribute to the animal model of ICV-STZ-induced AD-like manifestations. Further, drugs targeting to manage mitochondrial calcium homeostasis could best be studied in this animal model. Keywords Streptozotocin · Mitochondria · Calcium · memory · Sodium-calcium exchanger · Apoptosis
Introduction Alzheimer’s disease (AD) is a progressive neurodegenerative disorder with cardinal features such as memory loss, disorientation and gradual deterioration of intellectual ability. Recent reports suggest that almost 80% of the general public are concerned about developing dementia at some point of their lifetime and 1 in 4 people accept that dementia cannot be prevented. It is also projected that approximately 50 million people will suffer from AD throughout the globe. Moreover, about 75% caregivers of these patients express Communicated by Sreedharan Sajikumar. * Debapriya Garabadu [email protected]; [email protected] 1
Division of Pharmacology, Institute of Pharmaceutical Research, GLA University, Mathura 281 406, India
their difficulty to care and meet other responsibilities of life (Alzheimer’s Disease Interna
Data Loading...
