Mitochondrial Bioenergetics Methods and Protocols
Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic perform
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
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Mitochondrial Bioenergetics Methods and Protocols Edited by
Carlos M. Palmeira MitoLab, Department of Life Sciences, Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal
António J. Moreno Department of Life Sciences and IMAR, University of Coimbra, Coimbra, Portugal
Editors Carlos M. Palmeira MitoLab Department of Life Sciences Center for Neurosciences and Cell Biology University of Coimbra Coimbra, Portugal [email protected]
António J. Moreno Department of Life Sciences and IMAR University of Coimbra Coimbra, Portugal [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-381-3 e-ISBN 978-1-61779-382-0 DOI 10.1007/978-1-61779-382-0 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011938460 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface The purpose of this book is to provide a definitive treatise on the conceptual principles and practical considerations regarding research initiatives in mitochondrial function. There has been a robust emergence of interest in mitochondrial bioenergetics of late, much of it driven by realization of the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age. These initiatives have fostered the investment of enormous resources into the investigation of genetic and environmental influences on bioenergetics, demanding a certain degree of understanding of the fundamental principles and a level of proficiency in the practice of mitochondrial bioenergetic research. This book is intended as a bench reference for a broad scope of readers, spanning students of mitochondrial bioenergetics to practitioners in the industries of pharmaceutical and environmental sciences as well as mitochondrial genetics. Each section is prefaced with a short treatise introducing the fundamental principles for that section followed by chapters desc
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