Intravital Imaging of Dynamic Bone and Immune Systems Methods and P
This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertakin
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Masaru Ishii Editor
Intravital Imaging of Dynamic Bone and Immune Systems Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Intravital Imaging of Dynamic Bone and Immune Systems Methods and Protocols
Edited by
Masaru Ishii Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka, Osaka, Japan
Editor Masaru Ishii Department of Immunology and Cell Biology Graduate School of Medicine, Osaka University Osaka, Osaka, Japan
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7761-1 ISBN 978-1-4939-7762-8 (eBook) https://doi.org/10.1007/978-1-4939-7762-8 Library of Congress Control Number: 2018933038 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface During the last decade, advanced intravital imaging technology by using multiphoton excitation fluorescent microscopy has revolutionized the field of biological sciences. Based on these techniques, we are now able to visualize in situ behavior of a diversity of living cells “intravitally” within intact tissues and organs. This research trend would be quite meritorious especially for analyzing bone and immune systems, where various kinds of cell types are moving around and their spatiotemporal control is pivotal for proper functions in vivo. For example, bone is a mysterious organ where various kinds of hematopoietic and immune cells are produced and functioning altho
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