Nanoscale Imaging Methods and Protocols
This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biom
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Yuri L. Lyubchenko Editor
Nanoscale Imaging Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Nanoscale Imaging Methods and Protocols
Edited by
Yuri L. Lyubchenko Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE, USA
Editor Yuri L. Lyubchenko Department of Pharmaceutical Sciences University of Nebraska Medical Center Omaha, NE, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8590-6 ISBN 978-1-4939-8591-3 (eBook) https://doi.org/10.1007/978-1-4939-8591-3 Library of Congress Control Number: 2018944128 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer ScienceþBusiness Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Nanoimaging: Emerging Field in Biomedicine Nanoimaging combines a set of experimental approaches which are the foundation for single-molecule biophysics (SMB). The significance of SMB is defined by the two major features. First, the biological systems, including cells, consist of a limited number of biological molecules or complexes, so their study required techniques capable of probing single molecules. Second, upon functioning, biological molecules and systems assemble in transient states and characterization of these transient states is critical for understanding molecular mechanism of the biological process under study. Currently, SMB nanoimaging is applied to numerous biological problems associated with understanding molecula
