Investigation of Antimicrobial Peptide Salts: Efficacy and Solubility in Surfactant Solutions for Latex Systems
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0894-LL04-09.1
Investigation of Antimicrobial Peptide Salts: Efficacy and Solubility in Surfactant Solutions for Latex Systems Douglas A. Wicks1, Alicyn M. Rhoades1, John S. Williamson2 and Bruhaspathy Miriyala3; 1School of Polymers and High Performance Materials, The University of Southern Mississippi, Hattiesburg, Mississippi; 2Department of Medicinal Chemistry, The University of Mississippi, Oxford, Mississippi. 3Florous Technologies Inc., 970 William Pitt Way, Pittsburgh PA, 15238 ABSTRACT The study and application of antimicrobial peptides is a growing niche field in the areas of pharmaceutics and medicinal chemistry. As a result, the majority of testing and development is completed under in vitro conditions. The use of synthetically derived antimicrobial peptides in applications outside the medical realm is a relatively untapped field, with the significance of interactions between antimicrobial oligopeptides and the ingredients of polymer systems being largely unknown. Water-based polymer coatings systems are attractive targets for microbial invasion because of their inherent material properties. Water-based polymer latex coatings contain a number of components which aide in stabilization and coalescence of the polymer particles, such as surfactants and polymeric cellulose-derived molecules, with several types of molecular structures existing for each. Microbes are able to flourish within the water phase of the latexes while taking sustenance from these dispersion components, resulting in a loss of properties commonly known as ‘spoilage’. This work addresses the impact of formulation variables, specifically surfactant structure, on the solution and antimicrobial behavior of a model antimicrobial oligopeptides through the use of HT absorbance measurements. INTRODUCTION In latex polymer production, biocide molecules are often added post-polymerization to inhibit microorganism propagation.[1] Previous work within our research group has shown the reality of interactions between surfactant and biocide molecules in solution, and that the chemical nature of the surfactant impacts resulting efficacy of the biocide molecules.[2] In 1996, Blondelle and co-workers undertook a major combinatorial chemistry study to synthesize nearly 50 million different hexapeptides. Within that library, the most active structure was determined to be Ac-RRWWRF-NH2.[3] This peptide, designated Combi1, is a potent antimicrobial peptide with an IC50 activity against gram negative bacteria reported as low as 5 μg/mL.[3] Jing and coworkers determined that this peptide has a large influence on the thermotropic phase behavior of model bacteria membranes containing the negatively charged headgroup phosphatidylglycerol, but did not significantly interact with model erythrocyte membranes.[4] Jing et al also determined that the peptide interacts in solution with sodium dodecyl sulfate (SDS) surfactant micelles, however they did not determine the impact of such interaction on the
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antimicrobial behavior of Ac-RRWWRF-NH2. From the pe
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