Investigation of Changes in Exosomes Profile During Storage Period of Erythrocyte Suspensions
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ORIGINAL ARTICLE
Investigation of Changes in Exosomes Profile During Storage Period of Erythrocyte Suspensions Mehrdad Pashazadeh1
•
Haluk Barbaros Oral2 • Ferah Budak2
Received: 16 January 2020 / Accepted: 11 August 2020 Ó Indian Society of Hematology and Blood Transfusion 2020
Abstracts This study aimed to investigate the relationship of exosomes in erythrocyte suspension (ES) with leukoreduction and storage period. In this study, we expected to obtain new information about the immunomodulatory effect of allogeneic blood transfusion. Whole blood from healthy donors (10) were transferred to two separate blood bags by an equal amount converted into ES. One of the bags was passed through the leukocyte filter to obtain reduced leukocyte and non-reduced ESs and divided into four equal blood bags. Flow cytometry was used to identify and quantify exosomes in the samples on the 0th, 14th, 28th, and 42nd days at 4 °C. The exosomes were first coated with anti-CD9 antibodies with carboxylates beads and exosome volumes were determined by BCA Protein Assay to determine the amount of exosome-containing samples to be bound by beads and investigated by flow cytometry. Exosomes which were derived from Th, NK, NK-T cells were not detected in our study. There were no significant differences in exosome profiles between NL-ES and LR-ES groups. Statistically significant results (in comparison with the 0th day) were found as below. Exosomes that were derived from; T lymphocytes, decrease in LR-ES groups at 42nd-day samples (P \ 0.05). Tc lymphocytes, decrease in NL-ES groups at 14th day and 42ndday samples (P \ 0.05). B lymphocytes, a decrease in NLES groups at the sample of 28th and 42nd-days (P \ 0.05). MDSC and G-MDSC, decrease in both NL-ES groups and & Mehrdad Pashazadeh [email protected] 1
Medical Immunology, Faculty of Medicine, Bursa Uludag University, Bursa, Turkey
2
Department of Immunology, Faculty of Medicine, Bursa Uludag University, Bursa, Turkey
LR-ES groups at the sample of 14th, 28th, and 42nd-days (P \ 0.05). Our results suggest that exosomes in erythrocyte suspensions are not affected by the leukoreduction procedure. Exosomes which can freely pass from leukocyte filters may be the main cause of the insufficiency of leukoreduction to prevent TRIM. Keywords TRIM Immunomodulation Transfusion Leukoreduction Exosome
Introduction Extracellular vesicles (EVs) are composed of both microparticles produced by reverse budding of multimembrane bodies, spilling from the plasma membrane and released from nanovesicles or exosomes. Micro-granules are produced directly from the cell plasma membrane by external cell budding [1]. Although the release of apoptotic bodies during apoptosis has long been known [2], the fact that perfectly healthy cells shed vesicles from plasma membranes has only recently been appreciated. Most cells, including normal and diseased cells, can release the bilayer membrane-bound nanovesicles into the extracellular space and body fluids. Vesicles derived from this membrane (often r
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