Iron and zinc homeostases in female rats with physically active and sedentary lifestyles
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Iron and zinc homeostases in female rats with physically active and sedentary lifestyles Andrew J. Ghio . Joleen M. Soukup . Caroline Ghio . Christopher J. Gordon . Judy E. Richards . Mette C. Schladweiler . Samantha J. Snow . Urmila P. Kodavanti
Received: 3 March 2020 / Accepted: 28 October 2020 Ó This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020
Abstract To determine the effects of repeated physical activity on iron and zinc homeostases in a living system, we quantified blood and tissue levels of these two metals in sedentary and physically active Long-Evans rats. At post-natal day (PND) 22, female rats were assigned to either a sedentary or an active treatment group (n = 10/group). The physically active rats increased their use of a commercially-constructed stainless steel wire wheel so that, by the end of the study (PND 101), they were running an average of 512.8 ± 31.9 (mean ± standard error) min/night. After euthanization, plasma and aliquots of liver,
A. J. Ghio (&) J. M. Soukup C. Ghio Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Chapel Hill, NC 27599, USA e-mail: [email protected] C. J. Gordon Toxicity Assessment Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA J. E. Richards M. C. Schladweiler S. J. Snow U. P. Kodavanti Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA
lung, heart, and gastrocnemius muscle were obtained. Following digestion, non-heme iron and zinc concentrations in plasma and tissues were measured using inductively coupled plasma optical emission spectroscopy. Concentrations of both non-heme iron and zinc in plasma and liver were significantly decreased among the physically active rats relative to the sedentary animals. In the lung, both metals were increased in concentration among the physically active animals but the change in zinc did not reach significance. Similarly, tissue non-heme iron and zinc levels were both increased in heart and muscle from the physically active group. It is concluded that repeated physical activity in an animal model can be associated with a translocation of both iron and zinc from sites of storage (e.g. liver) to tissues with increased metabolism (e.g. the lung, heart, and skeletal muscle). Keywords Muscle
Exercise Iron Zinc Ferritins Liver
Abbreviations EDTA Ethylenediaminetetraacetic acid ICPOES Inductively coupled plasma optical emission spectroscopy MCH Mean corpuscular hemoglobin MCV Mean corpuscular volume MCHC Mean corpuscular hemoglobin concentration
123
Biometals
PND RBC TCA
Post-natal day Red
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