Isolation and analysis of fluP , a gene associated with hyphal growth and sporulation in Aspergillus parasiticus
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O R I GI N A L P A P E R
R. Zhou á R. Rasooly á J. E. Linz
Isolation and analysis of ¯uP, a gene associated with hyphal growth and sporulation in Aspergillus parasiticus
Received: 2 November 1999 / Accepted: 16 July 2000 / Published online: 1 September 2000 Ó Springer-Verlag 2000
Abstract A¯atoxins (AF) are polyketide-derived mycotoxins that frequently contaminate food and feed crops, causing health risks to animals and humans. The ¯uP gene was cloned by screening an Aspergillus parasiticus genomic DNA library with a cDNA probe encoding part of a polyketide synthase (PKS), the 6-methylsalicylic acid synthase (MSAS) from Penicillium patulum. FluP was hypothesized to function as a PKS in AF biosynthesis. The predicted amino acid sequence of FluP demonstrated a high degree of identity to MSAS (55%), moderate identity to another fungal PKS protein encoded by wA from A. nidulans (22%) and low identity (
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