Isolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potentia
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TECHNICAL NOTE
Isolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potential use in range-wide population studies Yoshimi Shinmura • Alison K. S. Wee • Koji Takayama • Sankararamasubramanian Halasya Meenakshisundaram • Takeshi Asakawa • Onrizal • Bayu Adjie • Erwin Riyanto Ardli • Sarawood Sungkaew • Norhaslinda Binti Malekal • Nguyen Xuan Tung Severino G. Salmo III • Orlex Baylen Yllano • M. Nazre Saleh • Khin Khin Soe • Emiko Oguri • Noriaki Murakami • Yasuyuki Watano Shigeyuki Baba • Edward L. Webb • Tadashi Kajita
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Received: 11 May 2012 / Accepted: 14 May 2012 Ó Springer Science+Business Media B.V. 2012
Abstract A set of 14 new microsatellite markers was developed for mangrove species Rhizophora mucronata (Rhizophoraceae) by using pyrosequencing. Fifty-six samples from 9 populations of R. mucronata in the Indo-West Pacific
region were genotyped; all loci were polymorphic, with the number of alleles ranging from 2 to 9. The mean expected heterozygosity per locus was 0.16 in a population from Sabah, no significant linkage disequilibrium was found among loci,
Y. Shinmura T. Asakawa Y. Watano T. Kajita (&) Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan e-mail: [email protected]
N. B. Malekal Institute for Tropical Biology and Conservation, Universiti Malaysia Kota Kinabalu, Kota Kinabalu, Malaysia
A. K. S. Wee E. L. Webb Department of Biological Sciences, National University of Singapore, Singapore, Singapore K. Takayama Department of Systematic and Evolutionary Botany, University of Vienna, Vienna, Austria S. H. Meenakshisundaram Biotechnology Programme, M.S. Swaminathan Research Foundation, Chennai, India Onrizal Forestry Sciences Department, Universitas Sumatera Utara, Medan, Indonesia B. Adjie Bali Botanical Garden, Indonesian Institute of Sciences, Bali, Indonesia E. R. Ardli Faculty of Biology, Jenderal Soedirman University, Purwokerto, Indonesia S. Sungkaew Forest Biology Department, Faculty of Forestry, Kasetsart University, Bangkok, Thailand
N. X. Tung Mangrove Ecosystem Research Centre, Hanoi National University of Education, Hanoi, Vietnam S. G. Salmo III Department of Environmental Science, School of Science and Engineering, Ateneo de Manila University, Quezon City, Philippines O. B. Yllano Biology Department, College of Sciences and Technology, Adventist University of the Philippines, Cavite, Philippines M. Nazre Saleh Department of Forest Production, Faculty of Forestry, Universiti Putra Malaysia, Serdang, Selangor, Malaysia K. K. Soe Department of Botany, University of Yangon, Yangon, Myanmar E. Oguri N. Murakami Makino Herbarium, Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan S. Baba Tropical Biosphere Research Center, University of the Ryukyus, Iriomote, Okinawa, Japan
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and significant deviation from Hardy–Weinberg equilibrium was found in 3 loci. The polymorphic microsatellite
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