Development and characterization of 14 microsatellites for the eastern chipmunk, Tamias striatus
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Development and characterization of 14 microsatellites for the eastern chipmunk, Tamias striatus Katherine Vandal1 · Carolyne Houle2 · Annie Archambault3 · Denis Réale1 · Dany Garant2 Received: 16 March 2020 / Accepted: 11 June 2020 © Springer Nature B.V. 2020
Abstract The number of microsatellite markers currently available for the eastern chipmunk Tamias striatus provides limited capacity to achieve sufficient pedigree building for the study of their genetic structure and relatedness patterns. We developed microsatellite loci for the eastern chipmunk, a small rodent commonly found in eastern North America. More specifically, we report data for 14 loci and 50 individuals genotyped from a population in southern Québec, Canada. We found a number of alleles ranging from 5 to 21 and there was no linkage disequilibrium among locus. One locus deviated from Hardy–Weinberg equilibrium and had a high proportion of null alleles. Those loci will be used in addition to previously developed loci to improve the precision of parentage assignment and population genetics studies on this species. Keywords Tamias striatus · Microsatellites · Population genetics · Parentage assignment
Introduction Eastern chipmunks (Tamias striatus) are small diurnal rodents ubiquitous of deciduous forests in eastern North America [1]. They are central-place foragers that carry resources back to their nest and rely on the availability of American beech (Fagus grandifolia) seeds for storage [2]. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05586-1) contains supplementary material, which is available to authorized users. * Katherine Vandal [email protected] Carolyne Houle [email protected] Annie Archambault [email protected] Denis Réale [email protected] Dany Garant [email protected] 1
Département des Sciences Biologiques, Université du Québec à Montréal, Montreal, QC, Canada
2
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada
3
Céréla Inc, St‑Hugues, QC, Canada
This tree species is characterized by large interannual variation in its seed production [masting events, [3]. Reproduction and related activities in northern populations of eastern chipmunks are tightly linked to masting events [4]. The eastern chipmunk is thus an ideal model to study the effects of variable food availability on animal populations. A longterm study of a wild population has been ongoing since 2004 in southern Québec, using 11 microsatellite loci developed by Anderson et al. [5] and Peters et al. [6]. Those microsatellites have enabled a better understanding of reproductive success patterns and pedigree structure [7, 8], as well as studies of dispersal and population genetics in this species [9, 10]. However, these markers provided limited capacity to achieve parental assignment when both parents were unknown based on social information alone, thus limiting the number
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