Isolation and Purification of Phenolic Acids from Sugarcane ( Saccharum officinarum L.) Rinds by pH-Zone-Refining Counte
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Isolation and Purification of Phenolic Acids from Sugarcane (Saccharum officinarum L.) Rinds by pH-Zone-Refining Counter-Current Chromatography and Their Antioxidant Activity Evaluation Ying-Tong Fang 1 & Quan Li 1 & Ao-Cheng Cao 2 & Yuan Li 2 & Yun Wei 1
Received: 2 November 2016 / Accepted: 17 January 2017 # Springer Science+Business Media New York 2017
Abstract The phenolic acids exhibit significant antioxidant, antiinflammatory, antimutation, and tyrosinase inhibitory effects. A high efficient and specific method for separation of phenolic acids from the sugarcane rinds based on pH-zonerefining counter-current chromatography technique was established. The separation was performed with a solvent system of methyl tert-butyl ether/acetonitrile/water at a volume ratio of 4:1:5, where 5 mM trifluoroacetic acid was added to the upper phase as a retainer and 3 mM NH4OH was added to the lower phase as an eluter. As a result, three phenolic acids including 4.8 mg of caffeic acid, 12.9 mg of ferulic acid, and 65.2 mg of p-coumaric acid were successfully purified in one run from 1.00 g crude extract with the purities of 93.4, 94.6, and 98.8%, respectively. 2,2-Diphenyl-1-picrylhydrazyl radical and total antioxidant capacity assay were used to evaluate antioxidant activities. The separated individual phenolic acid had higher antioxidant activity than their mixture. Caffeic acid
* Yun Wei [email protected] Ying-Tong Fang [email protected] Quan Li [email protected] Ao-Cheng Cao [email protected] Yuan Li [email protected] 1
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, People’s Republic of China
2
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, People’s Republic of China
showed the greatest antioxidant activity, followed by ferulic acid and p-coumaric acid. Keywords Phenolic acids . Sugarcane rinds . pH-zone-refining counter-current chromatography . Antioxidant activity
Introduction Sugarcane (Saccharum officinarum L.) is a principal raw material for the sugar industry, and cane sugar is 70% of the world’s sugars (Abbas et al. 2014). Planting area of sugarcane increased rapidly in the global harvest. A significant amount of residues including waste rinds and bagasses derive from the processing and harvesting of sugarcane (Attard et al. 2015). Sugarcane rinds which are generated in large quantities as waste byproduct of the sugar industry or ethanol production are approximately 20% weight of sugarcanes (Feng et al. 2015). Previous studies have shown that sugarcane contained a lot of functional compounds, such as phenolic acids. Waste sugarcane rinds also contain many phenolic acids and could be used as a good source of phenolic acids (Duarte-Almeida et al. 2011). Phenolic acids as antioxidants have been reported to possess potential protective effect against oxidative damage diseases, such as cancers, stroke, and coronary heart disease (Kukula-Koch et al. 20
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