Isolation of Cellulose-Degrading Bacteria and to Use Their Cellulolytic Potential for Production of Bioethanol from Pape
In the present scenario, solid waste management has emerged as a huge problem for the environment and mankind. Most of the wastes are generated from the plant source that is made up of cellulose. Cellulose can serve as the source of glucose by various met
- PDF / 445,800 Bytes
- 9 Pages / 439.37 x 666.142 pts Page_size
- 33 Downloads / 188 Views
Abstract In the present scenario, solid waste management has emerged as a huge problem for the environment and mankind. Most of the wastes are generated from the plant source that is made up of cellulose. Cellulose can serve as the source of glucose by various methods of degradation that can be chemical or enzymatic. The cellulase enzyme of the bacterial system can also be used for cellulose degradation. In the present study, cellulose-degrading bacteria have been isolated from soil obtained from wood habitat. To indicate the cellulase activity of the organisms, the diameter of clear zone around the colony and hydrolytic value on cellulose Congo Red agar media were measured. The strain with maximum cellulolytic potential was selected on the basis of DNS assay. The selected strain was further characterized at the molecular level using 16S RNA sequencing. The characterized strain was later used for the production of glucose by degrading the tissue paper. The glucose such produced was used for the production of bioethanol using Saccharomyces cerevisiae. The production of ethanol was positively tested after 6 days by iodoform test and amount of alcohol produced was obtained by distillation of the fermented mash. Keywords Cellulose · Cellulolytic bacteria · Paper waste · Bioconversion · Bioethanol
S. Chatterjee · K. Tripathi · R. S. Purty (B) University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi, India e-mail: [email protected] S. Chatterjee e-mail: [email protected] K. Tripathi e-mail: [email protected] © Springer Nature Singapore Pte Ltd. 2021 D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology, Lecture Notes in Bioengineering, https://doi.org/10.1007/978-981-15-7409-2_1
3
4
S. Chatterjee et al.
1 Introduction Human activities create wastes and these wastes are handled, stored, collected, and disposed of so that they do not become a risk for the health and environment. With the rapid increase in urbanization and population, the nature of solid waste has become complexed. Municipal solid waste (MSW) is generally categorized as degradable and nondegradable (Mehta et al. 2018). Wastes like paper, textile, fiber, food all those composed of plant components are degradable in nature and other wastes like plastic, metals, glass, e-wastes are termed as nondegradable. Characterization of MSW indicated that the waste consists of 30–45% organic matter, 6–10% recyclables, and the rest as inert matter (Kumar et al. 2009). The nature of waste suggests that the main content of waste is cellulose, which is the component of plant cell wall. Cellulose is a complex carbohydrate or polysaccharide consisting of several glucose units linked together by β-1,4 glycosidic bond (Klemm et al. 2005). Abundant availability and accessibility of cellulose make it a long-term raw material for producing many industrially important commercial products that will be costeffective and eco-friendly. But in the present juncture, much of the cellulose are disposed of as waste all ove
Data Loading...
