Molecular characterization of cellulolytic (endo- and exoglucanase) bacteria from the largest mangrove forest (Sundarban
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Annals of Microbiology
ORIGINAL ARTICLE
Open Access
Molecular characterization of cellulolytic (endo- and exoglucanase) bacteria from the largest mangrove forest (Sundarbans), Bangladesh Satyajit Biswas1†, Md. Al Saber1†, Ismoth Ara Tripty1, Md. Adnan Karim1,2, Md. Aminul Islam1, Md. Shazid Hasan3, A. S. M. Rubayet Ul Alam3, Md. Iqbal Kabir Jahid3 and Md. Nazmul Hasan1*
Abstract Purpose: Cellulase, due to its massive applicability, has been used in various industrial processes such as biofuels (bioethanol, triphasic biomethanation), agricultural and plant waste management, chiral separation, and ligand binding studies. The finding of a novel cellulase-producing bacterium will benefit the industries, which rely on yeast to produce cellulase in fermentation technology, because bacteria can easily be manipulated and fermented cost-effectively. Methods: Cellulase enzyme-secreting bacteria were isolated from different regions of the world’s largest mangrove forests, Sundarbans in Bangladesh. Biochemical, morphological, and 16S rRNA identification protocol was followed to precisely characterize the bacterial strains. Result: We have determined that the strain T2-D2 (Bacillus sp.), E1-PT (Pseudomonas sp.), and D1-PT (Pseudomonas sp.) showed maximum endoglycolytic and strain C1-BT (Bacillus sp.), E1-BT (Bacillus sp.), and T-4 (E) showed relatively higher exoglycolytic activity during the test. So, it can be easily cultured at a normal temperature (97.7–99.5 °F). On the one hand, T2D2 (Bacillus sp.) and E1-PT (Pseudomonas sp.) have shown the highest growth rate at pH 7 as it was neither acidic nor basic. Conclusion: It was concluded that the strain T2-D2 (Bacillus sp.) and E1-PT (Pseudomonas sp.) would be our target cellulolytic strains wherein the experimental isolates belonged to the Enterobacteriaceae, Psuedomonacea, Bacillacea, and Morganellacea family. Keywords: Cellulolytic bacteria, Endoglucanase, Exoglucanase, Mangrove forest, Bangladesh
Introduction Mangrove forests are enriched with groups of trees and shrubs and have substantial ecological importance at the tropical side and economic significance on universal scales. One of the largest mangrove forests in the world is Sundarbans located in the south-west between the river Baleswar * Correspondence: [email protected] † Satyajit Biswas and Md. Al Saber contributed equally to this work. 1 Laboratory of Pharmaceutical Biotechnology, Department of Genetic Engineering and Biotechnology, Faculty of Biological Science and Technology, Jashore University of Science and Technology, Jashore 7408, Bangladesh Full list of author information is available at the end of the article
in the East and the Harinbanga in the West, adjoining to the Bay of Bengal (Biswas et al. 2007). Having a rich biological diversity among the plants, animals, and microbes, Sundarbans is surrounded by terrestrial and marine ecosystems. To maintain the ecological balance and the biological diversity among the living material such as plant derived parts like litter, wood, leaf, stems, and so on
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