Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid
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Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) ISSN 1673-1581 (Print); ISSN 1862-1783 (Online) www.jzus.zju.edu.cn; www.springerlink.com E-mail: [email protected]
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Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid*# Wen-na BAO†1,2, Yi CHEN1, Hong-xiu LIAO1, Hang CHEN1, Shi-wang LIU1,2, Yong LIU1,2 1
School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China 2 Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Products, Hangzhou 310023, China † E-mail: [email protected] https://doi.org/10.1631/jzus.B2000269
The L(+)-form of tartaric acid (L(+)-TA) exists extensively in nature, and is widely used in the food, chemical, textile, building, and pharmaceutical industries (Su et al., 2001). The main method for L(+)TA production is microbial transformation by cisepoxysuccinate hydrolase (CESH), which can catalyze the asymmetric hydrolysis of cis-epoxysuccinic acid or its salts to TA or tartrate (Bao et al., 2019). Seventeen species containing CESH have been isolated so far. However, most species for L(+)-TA production have been reported from bacteria (Xuan and Feng, 2019). The only fungus isolated from soil by our lab recently, that could be used as catalyst for the process under acidic condition, is Aspergillus niger WH-2 (Bao et al., 2020). In order to find strains with new characteristics, this study attempted to isolate a new CESH source from fungi and investigate its application value. Unlike the previous isolation from soil, where the screening medium used contained a low concentration *
Project supported by the Zhejiang Provincial Natural Science Foundation of China (No. LQ19C200001) and the Zhejiang Xinmiao Talent Project (No. 2020R415014), China # Electronic supplementary materials: The online version of this article (https://doi.org/10.1631/jzus.B2000269) contains supplementary materials, which are available to authorized users ORCID: Wen-na BAO, https://orcid.org/0000-0003-3452-7938 © Zhejiang University and Springer-Verlag GmbH Germany, part of Springer Nature 2020
of substrate disodium cis-epoxysuccinate (99.6 This study expansum disodium WH-3 CESH Aspergillus Soil 1278 50 95 >99.6 Bao et al., niger WH-2 2020 Rhodococcus Soil >10 000 37 99 >99.5 Liu et al., opacus 2007 ML-0004 50 99 >99.5 Cheng et al., Klebsiella sp. Soil >10 000 2014 BK-58 Labrys sp. Soil 3597 55 99 >99.5 Bao et al., BK-8 2019 CESH: cis-epoxysuccinate hydrolase; EE: enantiomeric excess
Bao et al. / J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2020 21(10):835-840
function under acidic conditions, the substrate solution needs to be adjusted to neutral with the administration of high volumes of sodium carbonate, increasing the load on the ion exchange column. However, CESH from P. expansum WH-3 retains catalytic function at pH 4.5, indicating that it can be directly transformed without adjusting the pH. This reduces the requirement for sodium carbonate greatly, thereby
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