Uncovering proteomics changes of Penicillium expansum spores in response to decanal treatment by iTRAQ
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ORIGINAL ARTICLE
Uncovering proteomics changes of Penicillium expansum spores in response to decanal treatment by iTRAQ Ting Zhou 1 & Bishun Ye 2 & Zhiqian Yan 1 & Xiaohong Wang 2 & Tongfei Lai 2 Received: 22 March 2019 / Accepted: 6 January 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Abstract Exogenous decanal can significantly inhibit the germination and development of postharvest pathogen Penicillium expansum in vitro. Through an iTRAQ-based analysis, a global view of proteomic alteration of P. expansum spores under decanal treatment was acquired. A total of 246 up-regulated and 293 down-regulated differentially expressed proteins (DEPs) were identified. Among them, DEPs related to glutathione metabolism, ribosome, and oxidative phosphorylation pathway were noticed for their functional significance, large number and high rich value in pathway enrichment statistics. Further analysis found that, under decanal stress, expression of 9 genes corresponding to DEPs involved in the oxidative phosphorylation pathway showed a significantly decreasing trend, and activities of 3 crucial enzymes (NADH dehydrogenase, CoQ-Cytochrome c reductase, and F1F0-ATP synthetase) were inhibited. Also, a decreased in ATP content, reduction in the number of mitochondria, and weakening in carbohydrate consumption were detected. Based on these results, disturbance of oxidative phosphorylation would partly be responsible for the inhibitory effect of decanal on the growth of P. expansum. The findings would provide new insights into exploring the possible antifungal mechanisms of decanal. Keywords Penicillium expansum . Decanal . iTRAQ . Oxidative phosphorylation
Introduction Penicillium expansum, as a necrotrophic pathogen, can infect a wide range of fruits, especially pome and stone fruits (Li et al. 2015). P. expansum can lead huge economic losses and threaten human health due to the production of a range of mycotoxins (Barad et al. 2016). By far, synthetic chemical fungicide application is one of the primary methods for postharvest disease controlling. Many new fungicides showing Ting Zhou and Bishun Ye contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42161-020-00486-6) contains supplementary material, which is available to authorized users. * Tongfei Lai [email protected] 1
Hangzhou Key Laboratory for Safety of Agricultural Products, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China
2
Research Centre for Plant RNA Signaling, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China
great antifungal potential are continually designed and developed (Chen et al. 2018). However, the development of fungicidal resistance and growing public concerns over human health and environmental safety gradually limit its application (Morales et al. 2010). Therefore, developing eco-friendly alternative strategies for postharvest disease management is always necessary. Esse
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