Isolation of Plant Growth-Promoting Bacillus cereus from Soil and Its Use as a Microbial Inoculant
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RESEARCH ARTICLE-BIOLOGICAL SCIENCES
Isolation of Plant Growth-Promoting Bacillus cereus from Soil and Its Use as a Microbial Inoculant Ann Mary Sebastian1 · Mridul Umesh1 · Kumaresan Priyanka2 · Kathirvel Preethi2 Received: 26 June 2020 / Accepted: 18 August 2020 © King Fahd University of Petroleum & Minerals 2020
Abstract Modernization has introduced intensive agricultural practices wherein the pesticides play an important role both in stabilization and in increase of agricultural products. As a consequence, humans and members of other ecosystems are exposed to increased levels of compounds that have detrimental effects on their health, thereby signifying the importance of microbial inoculants. In order to achieve this goal 7 different bacterial species were initially screened for isolation of plant growth-promoting Bacillus sp. The isolate CUAMS116 was confirmed to be Bacillus cereus through biochemical and molecular characterization. The in vitro plant growth-promoting ability of the isolate was screened through standard tests. Different concentrations of bacterial inoculant (25%, 50%, 75%, 100%) were evaluated for its plant growth promotion ability using Phaseolus vulgaris L., under pot culture conditions. At the harvest stage, the mature control plants measured 16.53 cm and mean treated plant height was measured to be 27.75 cm, showing a maximum percentage increase in length of 67.87%. The results suggested that the B. cereus CUAMS116 isolated in this study can be extended as a PGPM through further field trials in other plants for improving crop yield and tolerance to biotic and abiotic stresses. Keywords Bacillus cereus · PGPM · Growth promotion · Bacterial inoculant · Phaseolus vulgaris
1 Introduction Rhizosphere encompasses the narrow zone of soil directly surrounding the root system of plants [1]. Groups of rhizosphere bacteria that are competent in colonizing the root environment are referred to as rhizobacteria [2]. The plants have evolved with a plenitude of micro-organisms with diverse functional potential [3]. Plant growth-promoting micro-organisms (PGPMs) are primarily found existing in the rhizosphere. They are characterized by the presence of different root secretions and intense microbial activity [4]. Root colonization involves one of the major processes which determines the survival of the bacteria after inoculating the Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13369-020-04895-8) contains supplementary material, which is available to authorized users.
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Mridul Umesh [email protected]
1
Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, Karnataka 560029, India
2
Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu 641105, India
seeds or soil. The bacterial multiplication happens in the spermosphere, in case of germinating seeds, stimulated by the carbohydrate and amino acid rich seed exudates [5]; similarly, they cling to the root surface and colonize the root syst
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