Isolation and evaluation of the plant growth promoting rhizobacterium Bacillus methylotrophicus (DD-1) for growth enhanc
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ORIGINAL PAPER
Isolation and evaluation of the plant growth promoting rhizobacterium Bacillus methylotrophicus (DD‑1) for growth enhancement of rice seedling Zeping Liu1,2 · Hengxu Wang1,2 · Weihui Xu1,2 · Zhigang Wang1,2 Received: 4 March 2020 / Revised: 28 May 2020 / Accepted: 1 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Bacillus methylotrophicus has been demonstrated to promote growth of various plants, whereas the promoting effect of B. methylotrophicus on rice growth has been rarely reported. In this study, B. methylotrophicus DD-1, capable of efficiently promoting the growth of rice, was isolated from the root soil of rice plants. The isolate exhibited potassium-solubilizing (1.18 mg/L), Indole-3-acetic acid (IAA) (87.26 mg/L), Gibberellic acid (GA) (25.91 mg/L) and Siderophore production activity (52.32%). As indicated from the result, plant growth parameters (e.g., dry weight, tiller number, root and shoot length) of rice seedlings treated with the isolate DD-1 were more effective than those of the control group in pot and soilless culture experiments. Moreover, the adsorption capacity of rice roots which were soaked in the bacterial suspension of isolate increased with the increase in concentration and absorption time. In sterilized and unsterilized soil, conformation of root colonization activity by bacterial isolate established by its nearer existence to the rice root. Thus, the B. methylotrophicus DD-1 enhances plant growth promotion by multifarious growth promoting and root colonization traits, thereby augmenting potassium level in soil. Henceforth, the potential bacterium could be exploited for the development of biological fertilizer, leading towards sustainable agronomy. Keywords Bacillus methylotrophicus · Rice · Growth-promoting · Potassium-solubilizing Abbreviations PGPR Plant growth promoting rhizobacteria IAA Indole-3-acetic acid GA Gibberellic acid CAS Chrom Azurol S LB Luria–Bertani
Communicated by Erko Stackebrandt. * Zhigang Wang [email protected] 1
School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, Heilongjiang, China
Heilongjiang Provincial Technology Innovation Center of Agromicrobial Preparation Industrialization, Qiqihar 161006, Heilongjiang, China
2
Introduction In China, rice refers to the grain crop that has been reported with the widest planting area and the highest total output. Nearly 60% of the population in this nation eat rice as the primary food (Zhang et al. 2017a, b). Rice is critical to China’s grain production and shows tight relationships to people’s livelihood (Yin et al. 2018). Though a wide variety of biotic and abiotic stresses can affect rice growth, soil fertility is found as one of the vital constraints affecting crop yield (Das et al. 2016). Potassium refers to one of the leading nutrient elements impacting rice growth and grain quality. Rice is capable of absorbing potassium by drawing upon soil minerals, organic matter and synthetic fertilizers (Kang et al. 2017). H
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