Isolation of saponin-free fraction from Ginseng ( Panax ginseng C.A. Meyer) and its effects on the function of neutrophi
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Isolation of Saponin-free Fraction from Ginseng (Panax ginseng Ca~. Meyer) and its Effects on the Function of Neutrophils Lioudmila Larina *~, Byung Goo Cho**, Leonid Ten** and Hoon Park*'* Institute of Biochemical Physics, Russian Academy of Sciences~ 117977 Moscow, Kosigin St. 4, Russia *Korea Ginseng and "lbbacco Research Institute, 302 Shinsong-Dong, Science ~ Yusong-ku, Daeieon 305-345, Korea (Received t4 M~O' 2001 9 accepted 27 .hdy 200t)
Abstraet-A method of i~)Iation of bioactive ,a.ater-soluble saponin-free subti'action (RG-ws-l-l-l) li'om Korean red ginseng using solvent extraction, ultrafiltration and chromatography on Diaion HP-20 resin was developed. 3b e',,aluate the potency of RC~-ws-l-l-t to effect on the heat stress (HS) response and antMnflammatory response in ncutrophils, the oxygen consumption (OC) and Ibrmation of superoxide radicals (FSRt ~.ere assayed in suspensions of neutrophils li'om mice. Supplementation of RG-ws-l-l-I to mice in rh'o (200 mg/kg, 14 days intrapcritoneally) results in the increase OC by' neutrophils ass~,ed ex vivo at 37 "C. OC and FSR by neum~phils were suppressed when cells obtained fi'om the cxmtrol animals were incubated at 42 "('. Preineubation of neutmphits with RG-ws-l-I-1 reduces H S related depression of F SR. Supplementation of RG-ws-I-l-1 to mice in rh'o decreases ItS mlated suppression of OC. RG-ws-1-1-1 st~bli'action significm]tly improves aza oxygen-dependent anti-mi~obial function of neutmphils, al~ studied under the 14S conditions. Key words: Chromatographic Method, Ultrallltmtion, Ginseng, Neutrophils, fteat Stress
rameters) were 1,eported, Each of them has some advantages and imperfections [Emel'yanova et al., 19961. We proposed to use the functional model system, namely murine neutrophils with regard to their physiological functions. This cell system is quite suitable to evaluate anti-inflammatory and anti-heat stress effects of Korean red ginseng (KRG) supplemented either in vn,o or in vitro, In fact neutrophils are responsible for defense against pathogens. Neutrophils are the primary cells that are involved in the killing of bacteria via an oxygen-dependent anti-microbial system, thal is able to form strong oxidants - reactive oxygen species including superoxide ion (O:), hydrogen peroxide (H_,O,), hypochlorous acid (HOCI) and hydroxyl radical (Oil"), When nentmphils are exposed to microo~anisms, an increase in the rate of ~ consumption (the respiratory burst) is induced. An increased formation of reactive oxygen species is the consequence and strong oxidants like hypochlorous acid and H_~O_~are released into the intracellular space of neutrophik [Badwey and Kamovsky, 1980]. On the other hand, the exposure of neutrophik in suspensions as well as animals to elevated temwmtures results in the heat stress response (HSR) in ceils [Bornman et al., f998]. In particular, during the t IS a depression in the physiological functions of phagocytes is observed, including a decrease in consumption of oxygen and in the rate of formation ofsuperoxide radicals aRer
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