Arthrobacter ginsengisoli sp. nov., isolated from soil of a ginseng field
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Original Paper
Arthrobacter ginsengisoli sp. nov., isolated from soil of a ginseng field Muhammad Zubair Siddiqi · Yeon‑Ju Kim · Van‑An Hoang · Muhammad Hanif Siddiqi · Md. Amdadul Huq · Deok‑Chun Yang
Received: 29 January 2014 / Revised: 4 August 2014 / Accepted: 5 August 2014 © Springer-Verlag Berlin Heidelberg 2014
Abstract A Gram-staining-positive, catalase-positive, oxidase-negative, non-motile, non-flagellate and rodshaped bacterium, was designated as DCY81T, and isolated from soil of a ginseng field in Pocheon province, Republic of Korea. The 16S rRNA gene sequence analysis revealed that strain DCY81T belonged to the genus Arthrobacter. Major fatty acid was anteiso-C15:0, while major polar lipids were diphosphatidyglycerol, phatidyglycerol, phosphatidylinositol, monogalactosyldiacylglycerol (GL1), and dimannosyldiacylglycerol (GL2). The dominant quinone was MK-9(H2). The peptidoglycan type was A3α with an l-Lys–l-Ala–l-Thr–l-Ala interpeptide bridge. The DNA– DNA hybridization relatedness between strain DCY81T and Arthrobacter siccitolerans LMG 27359T (98.2 %), Arthrobacter sulfonivorans JCM 13520T (97.81 %), Arthrobacter
scleromae DSM 17756T (97.59 %), Arthrobacter oxydans KCTC 3383T (97.3 %) was 39.1 ± 0.2, 62.2 ± 1.6, 36.8 ± 1.1 and 48.3 ± 1.6 %, respectively which show that the genotypic separation of strain DCY81T from the closest reference strain of the genus Arthrobacter. The DNA G+C content was 65.2 mol%. The genotypic analysis, physiological, and chemotaxonomic results indicate that strain DCY81T represents a novel species of the genus Arthrobacter. Therefore, Arthrobacter ginsengisoli sp. nov., is proposed as the type strain (=KCTC 29225T = JCM 19357T). Keywords Actinobacteria · Arthrobacter ginsengisoli · Taxonomy · Ginseng soil
Introduction Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY81T is KF212463. Muhammad Zubair Siddiqi and Yeon-Ju Kim have contributed equally to this manuscript. Electronic supplementary material The online version of this article (doi:10.1007/s00203-014-1025-8) contains supplementary material, which is available to authorized users. M. Z. Siddiqi · Y.-J. Kim · V.-A. Hoang · M. H. Siddiqi · M. A. Huq · D.-C. Yang (*) Graduate School of Biotechnology, Ginseng Genetic Resource Bank College of Life Science, Kyung Hee University, Yongin 446‑701, Republic of Korea e-mail: [email protected] Y.-J. Kim e-mail: [email protected]
During the course of investigating bacterial diversity in the ginseng soil by culture-dependent methods, a large number of bacteria were isolated. At least 30 bacterial genera were found such as Arthrobacter, Achromobacter, Bacillus, Brevibacillus, Chryseobacterium, Delftia, Enterobacter, Epilithonimonas, Erwinia, Ewingella, Flavobacterium, Flexibacter, Klebsiella, Labrys, Microbacterium, Methylobacterium, Mucilaginibacter, Pedobacter, Polaromonas, Pseudomonas, Pusillimonas, Raoultella, Rhodococcus, Serratia, Solimonas, Sphingobacterium, Sphingomonas, Sphingopyxis, Sten
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