Isopanduratin A Isolated from Boesenbergia pandurata Reduces HepG2 Hepatocellular Carcinoma Cell Proliferation in Both M
Medicinal plants have been used for disease treatment throughout history, especially in parts of Asia. Vietnam is a tropical country which possesses forests with a wide diversity of plants, which have a long history of being used as alternative remedies f
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Isopanduratin A Isolated from Boesenbergia pandurata Reduces HepG2 Hepatocellular Carcinoma Cell Proliferation in Both Monolayer and Three-Dimensional Cultures Sinh Truong Nguyen, Nghia Minh Do, Duyen Ho-Khanh Tran, Ngoc Bao To, Phuc Hong Vo, Mai Thi Thanh Nguyen, Nhan Trung Nguyen, Hai Xuan Nguyen, Kiet Dinh Truong, and Phuc Van Pham Abstract
Introduction Medicinal plants have been used for disease treatment throughout history, especially in parts of Asia. Vietnam is a tropical country which possesses forests with a
wide diversity of plants, which have a long history of being used as alternative remedies for treatment of various diseases. In this study, we aimed to test the bioactivity of Boesenbergia pandurata (B. pandurata) plant extract-derived compounds, including
Sinh Truong Nguyen, Nghia Minh Do, and Phuc Hong Vo Stem Cell Institute, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam Cancer Research Laboratory, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam Vietnam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam e-mail: [email protected] Duyen Ho-Khanh Tran and Ngoc Bao To Stem Cell Institute, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam Vietnam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam e-mail: [email protected] Mai Thi Thanh Nguyen, Nhan Trung Nguyen, and Hai Xuan Nguyen Vietnam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam Faculty of Chemistry, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam e-mail: [email protected]
Kiet Dinh Truong Medical Genetic Institute, Ho Chi Minh City, Vietnam e-mail: [email protected] Phuc Van Pham (*) Stem Cell Institute, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam Cancer Research Laboratory, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam Vietnam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam Laboratory of Stem Cell Research and Application, University of Science Ho Chi Minh City, Ho Chi Minh City, Vietnam e-mail: [email protected]; [email protected]; [email protected]
Sinh Truong Nguyen et al.
Pinostropin, Pinocembrin, Alpinetin, and Isopanduratin A, on HepG2 hepatocellular carcinoma cells. Method In this study, several compounds from Boesenbergia pandurata plant extract, including Pinostropin, Pinocembrin, Alpinetin, and Isopanduratin A, were used to evaluate their antitumor effects on HepG2 hepatocellular carcinoma cells in both monolayer culture condition (2D culture) and three-dimensional culture condition (3D culture); HepG2 cell proliferation was assessed by Alamar Blue assay. The compound which had the strongest cytotoxicity was selected for further analysis by Propidium Iodide (PI) staining assay and caspase 3/7 expression assay. Results The cytotoxicity assay showed that Isopanduratin A had the strongest cytotoxic effect on HepG2 cells grown in both 2D culture (IC50 at 357 10 nM) and 3D culture conditions (IC50 at 744 18 nM). The PI staining of HepG2 spher
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