Jasmonic acid and hydrogen sulfide modulate transcriptional and enzymatic changes of plasma membrane NADPH oxidases (NOX
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Jasmonic acid and hydrogen sulfide modulate transcriptional and enzymatic changes of plasma membrane NADPH oxidases (NOXs) and decrease oxidative damage in Oryza sativa L. during thiocyanate exposure Xiao-Zhang Yu
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Yun-Peng Chu1 Hua Zhang1 Yu-Juan Lin1 Peng Tian1 ●
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Accepted: 10 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract It is evident that the plasma membrane NADPH oxidases (NOXs) play an important role in the generation of superoxide radicals (O2−•) in plants during defense responses. This study was to clarify activation of NOXs in oxidative damage in Oryza sativa during SCN− exposure, particularly in the roles of jasmonic acid (JA) and hydrogen sulfide (H2S) on transcriptional and enzymatic changes of NOXs. Results indicated that enzymatic activity of NOXs in both roots and shoots was significantly enhanced during SCN− exposure, whereas the application of JA and H2S donor (NaHS) significantly repressed NOXs activity in SCN-treated rice seedlings. Similarly, ROS analysis showed that SCN− exposure elevated the content of O2−•, hydrogen peroxide (H2O2) and malondialdehyde (MDA) in rice tissues significantly, whereas decreases in O2−• and H2O2 were detected in roots and shoots of SCN-treated rice seedlings due to application of JA and NaHS. PCR analysis revealed different expression patterns of 7 plasma membrane-localized NOX genes in rice roots and shoots against SCN− exposure, suggesting that various isogenes of NOXs might regulate and determine activity of NOXs in rice organs. In conclusion, SCN− exposure was able to trigger activation of NOXs effectively, and led to oxidative damage and lipid peroxidation; the effects of JA and NaHS on inactivation of NOXs was evident and tissue specific, which in turn modulated ROS accumulation in rice plants. Keywords Hydrogen sulfide Jasmonic acid NADPH oxidases Rice seedlings ROS Thiocyanate ●
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Introduction When plants suffer from various unfavorable stresses, one of earlier toxic signals is over accumulation of ROS in cellular organelles (Bartoli et al. 2004). ROS are one of the most important byproducts of various metabolic pathways (Gill and Tuteja 2010). It can be formed in protoplastic sources in plants, including chloroplast, mitochondria and peroxisomes (Bolwell et al. 2002; Navrot et al. 2007), because these cell compartments are having extremely oxidizing metabolic activity with a high electron flow (Gill and Tuteja 2010). Usually, ROS in plant cells is naturally
* Xiao-Zhang Yu [email protected] 1
College of Environmental Science & Engineering, Guilin University of Technology, 541004 Guilin, PR China
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maintained at very low level, which functions as an important signaling molecule to regulate growth and development of plants (Foreman et al. 2003). However, accumulation of ROS is detectable in plants that can cause oxidative burst under unfavorable environment (Xie et al. 2014). In fact, modification of protein, oxidation of lipid, and disturbance of membrane integrity o
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