Karyotypic mosaicism and molecular cytogenetic markers in the scleractinian coral Acropora pruinosa Brook, 1982 (Hexacor
- PDF / 1,222,397 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 73 Downloads / 184 Views
REPORT
Karyotypic mosaicism and molecular cytogenetic markers in the scleractinian coral Acropora pruinosa Brook, 1982 (Hexacorallia, Anthozoa, Cnidaria) Takahiro Taguchi1,2 • Erika Tagami3 • Takuma Mezaki4 • Joshua Magnaye Vacarizas1 • Katrina Lasalita Canon1 • Teresa Narvaez Avila1 • Dan Anthony UY Bataan1 • Akira Tominaga1 • Satoshi Kubota1
Received: 26 December 2019 / Accepted: 7 July 2020 Ó Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Molecular cytogenetic investigation was carried out on the scleractinian coral, Acropora pruinosa (A. pruinosa). Conventional Giemsa staining techniques for karyotyping, such as G- and C-banding, were conducted. Karyotype analysis showed mosaicism of cells with two different chromosome numbers, 28 and 29, in the same embryo. The C-band positive portions appeared on the centromeres of most chromosomes and along the entire length of the unpaired chromosome 15 in cells with 29 chromosomes. Fluorescence in situ hybridization (FISH) revealed that the loci of rRNA genes (rDNA), 5S and 18S/ 28S, were located on chromosomes 4 and 2, respectively. Whole genome hybridization (WGH) with sperm DNA showed distinct signals not only on all centromeric regions but also on a whole unpaired chromosome 15. Comparative genomic hybridization (CGH) using DNAs from sperm and unfertilized eggs revealed that the unpaired chromosome Topic Editor Morgan S. Pratchett
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00338-020-01975-x) contains supplementary material, which is available to authorized users. & Takahiro Taguchi [email protected] 1
Kuroshio Science Course, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi 783-8505, Japan
2
Department of Nutrition, Faculty of Health Sciences, Kochi Gakuen University, 292-26 Asahitenjin-cho, Kochi 780-0955, Japan
3
Clinical Trials Testing Section, Biologics, SRL Inc., Komiyacho, Hachioji, Tokyo 192-8535, Japan
4
Kuroshio Biological Research Foundation, Otsuki, Hata County, Kochi 788-0333, Japan
15 has sperm-specific DNA sequences. We therefore hypothesized the existence of a sex-related chromosome in A. pruinosa. In this study, we have presented a tentative karyotype of this coral, based on banding results, and described FISH, WGH, and CGH results. Moreover, we successfully cloned and sequenced three clones; AP-5S for 5S rDNA, AP-18S for 18S/28S rDNA, and AP-unpaired chromosome 15q for the unpaired chromosome 15-specific DNA. These molecular cytogenetic approaches will help establish a more exact karyotype of corals and promote understanding of coral genetics, including chromosome evolution. These findings will help to verify the method of classification of complex scleractinian corals. Keywords Scleractinian coral Acropora Mosaicism Chromosome FISH rRNA genes
Introduction Acropora is a genus of small-polyped stony coral in the phylum Cnidaria. Some of its species are known as table coral, elkhorn coral, and staghorn coral. Acropora spe
Data Loading...
