Endozoicomonas coralli sp. nov., isolated from the coral Acropora sp.
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ORIGINAL PAPER
Endozoicomonas coralli sp. nov., isolated from the coral Acropora sp. Wen‑Ming Chen1 · Kai‑Rou Lin1 · Shih‑Yi Sheu2 Received: 19 April 2018 / Revised: 25 October 2018 / Accepted: 26 October 2018 / Published online: 12 November 2018 © Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract A novel bacterium, designated strain Acr-12T, was isolated from the coral Acropora sp. off coast of Southern Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Acr-12T belonged to the genus Endozoicomonas and had closest phylogenetic identity to Endozoicomonas acroporae Acr-14T (98.7%) and Endozoicomonas atrinae WP70T (97.8%). Cells of strain Acr-12T were Gram-negative, aerobic, non-motile, poly-β-hydroxybutyrate-accumulating, rod-shaped and formed creamy white colonies. Optimal growth occurred at 30 °C, pH 7, and in the presence of 3% NaCl. Strain Acr-12T contained summed feature 3 (C16:1ω7c and/or C 16:1ω6c), summed feature 8 (C18:1ω7c and/or C 18:1ω6c) and C16:0 as the predominant fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The predominant isoprenoid quinone was Q-9. The genomic DNA G + C content was 49.6 mol%. The DNA–DNA relatedness of strain Acr-12T with respect to the closest species of the genus Endozoicomonas was less than 30%. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Endozoicomonas. On the basis of the genotypic, chemotaxonomic, and phenotypic data, strain Acr-12T represents a novel species in the genus Endozoicomonas, for which the name Endozoicomonas coralli sp. nov. is proposed. The type strain is Acr-12T (= BCRC 80921T = KCTC 42900T). Keywords Endozoicomonas coralli sp. nov. · Gammaproteobacteria · Oceanospirillales · Hahellaceae · Polyphasic taxonomy
Introduction
Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Endozoicomonas coralli strain Acr-12T is LN879492. The Digital Protologue database Taxon Number for strain Acr-12T is TA00505. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-018-1591-2) contains supplementary material, which is available to authorized users. * Shih‑Yi Sheu [email protected] 1
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, No. 142, Hai‑Chuan Rd. Nan‑Tzu, Kaohsiung 811, Taiwan
Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, No. 142, Hai‑Chuan Rd. Nan‑Tzu, Kaohsiung 811, Taiwan
2
The genus Endozoicomonas (type species, Endozoicomonas elysicola), first established by Kurahashi and Yokota (2007) and emended by Nishijima et al. (2013) and Pike et al. (2013), is a member of the family Hahellaceae, in the order Oceanospirillales (http://www.bacterio.net/-class ifphyla.html#hahellaceae; Garrity et al. 2005) of the class Gammaproteobacteria. At t
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