Label-Free Rapid Detection of Pathogens with Antimicrobial Peptide Assisted Impedance Spectrometry
- PDF / 185,700 Bytes
- 6 Pages / 432 x 648 pts Page_size
- 16 Downloads / 132 Views
/DEHO)UHH5DSLG'HWHFWLRQRI3DWKRJHQVZLWK$QWLPLFURELDO3HSWLGH$VVLVWHG ,PSHGDQFH6SHFWURPHWU\ .HUHQ-LDQJ+DVKHP(WD\DVK6DUIXGGLQ$]PL*DULPD7KDNXU6HOYDUDM1DLFNHU.DPDOMLW .DXUDQG7KRPDV7KXQGDW
'HSDUWPHQWRI&KHPLFDODQG0DWHULDOV(QJLQHHULQJ8QLYHUVLW\RI$OEHUWD(GPRQWRQ$OEHUWD
7*9&DQDGD
)DFXOW\ RI 3KDUPDF\ DQG 3KDUPDFHXWLFDO 6FLHQFHV 8QLYHUVLW\ RI $OEHUWD(GPRQWRQ$OEHUWD
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
13
EDFWHULDWKDWHQDEOHDZLGHUDQJHRIDSSOLFDWLRQV>@,QUHFHQW\HDUVVH
Data Loading...
