Leaf nitrate accumulation influences the photorespiration of rice ( Oryza sativa L.) seedlings
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Leaf nitrate accumulation influences the photorespiration of rice (Oryza sativa L.) seedlings Yuming Sun & Yingrui Li & Bo Wang & Yong Li & Lei Ding & Min Wang & Luis Alejandro Jose Mur & Xiaorong Fan & Qirong Shen & Shiwei Guo
Received: 19 February 2020 / Accepted: 7 September 2020 # Springer Nature Switzerland AG 2020
Abstract Aims The form of nitrogen (N) supply influences photorespiration in C3 plants, but whether nitrate (NO3−) regulates photorespiration and, if so, the underlying mechanisms for such regulation are still unclear. Methods Three hydroponic experiments were conducted in a greenhouse to investigate the relationships between leaf NO3− concentrations and photorespiration rates in rice (Oryza sativa L.) genotypes cv. ‘Shanyou 63’ hybrid indica and ‘Zhendao 11’ hybrid japonica or using mutants that overexpress NRT2.1 (in cv.
‘Nipponbare’ inbred japonica). We estimated photorespiratory rate from the CO2 compensation point in the absence of daytime respiration (Γ*) using the biochemical model of photosynthesis. Results Higher Γ* values under high N level or NO3− were significantly and positively correlated with leaf NO3− concentrations. Further elevating leaf NO3− concentrations by either resuming NO3− nutrition supply after N depletion (in cv. ‘Shanyou 63’ hybrid indica and ‘Zhendao 11’ hybrid japonica) or using mutants that overexpress NRT2.1 (in cv. ‘Nipponbare’ inbred japonica) increased
Yuming Sun and Yingrui Li contributed equally to this work. Responsible Editor: Ad C. Borstlap. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04710-1) contains supplementary material, which is available to authorized users. Y. Sun : Y. Li : B. Wang : L. Ding : M. Wang : X. Fan : Q. Shen : S. Guo (*) Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Centre for Organic-based Fertilizers, Jiangsu Collaborative Innovation Centre for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing 210095, China e-mail: [email protected]
Y. Sun e-mail: [email protected] Y. Li e-mail: [email protected]
B. Wang e-mail: [email protected] L. Ding e-mail: [email protected] M. Wang e-mail: [email protected] X. Fan e-mail: [email protected] Q. Shen e-mail: [email protected]
Plant Soil
Γ* values. Additionally, the activities of leaf nitrate reductase (Nr) and concentrations of organic acids involving in the tricarboxylic acid (TCA) cycle synchronously changed as environmental conditions were varied. Conclusions Photorespiration rate is related to the leaf NO3− concentration, and the correlation may links to the photorespiration-TCA derived reductants required for NO3− assimilation. Keywords Rice (Oryza sativa L.) . Ammonium . Nitrate . Photorespiration rate . Tricarboxylic acid cycle . Malic acid Abbreviations A net photosynthetic rate Ci intercellular CO2 concentration Ci* apparent CO2 compensation point in the absence of respiration gm mesophyll conductance gs st
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