Leptin and TNF-alpha promoter methylation levels measured by MSP could predict the response to a low-calorie diet
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ORIGINAL PAPER
Leptin and TNF-alpha promoter methylation levels measured by MSP could predict the response to a low-calorie diet Paul Cordero & Javier Campion & Fermin I. Milagro & Estibaliz Goyenechea & Thais Steemburgo & Biola M. Javierre & J. Alfredo Martinez
Received: 6 January 2011 / Accepted: 1 March 2011 / Published online: 5 April 2011 # University of Navarra 2011
Abstract Obesity-associated adipose tissue enlargement is characterized by an enhanced proinflammatory status and an elevated secretion of adipokines such as leptin and cytokines such as tumor necrosis factor (TNF)-alpha. Among the different mechanisms that could underlie the interindividual differences in obesity, epigenetic regulation of gene expression has emerged as a potentially important determinant. Therefore, 27 obese women (age, 32–50 years; baseline body mass index, 34.4±4.2 kg/m2) were prescribed an 8-week low-calorie diet, and epigenetic marks were assessed. Baseline and endpoint anthropometric parameters were measured, and blood samples were drawn. Genomic DNA and P. Cordero : J. Campion : F. I. Milagro : J. A. Martinez (*) Department of Nutrition and Food Sciences, Physiology and Toxicology, University of Navarra, Pamplona, Spain e-mail: [email protected] E. Goyenechea Donostia Hospital, San Sebastian, Spain
RNA from adipose tissue biopsies were isolated before and after the dietary intervention. Leptin and TNF-alpha promoter methylation were measured by MSP after bisulfite treatment, and gene expression was also analyzed. Obese women with a successful weight loss (≥5% of initial body weight, n=21) improved the lipid profile and fat mass percentage (−12%, p
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