LINC00657 knockdown suppresses hepatocellular carcinoma progression by sponging miR-424 to regulate PD-L1 expression
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Genes & Genomics https://doi.org/10.1007/s13258-020-01001-y
RESEARCH ARTICLE
LINC00657 knockdown suppresses hepatocellular carcinoma progression by sponging miR‑424 to regulate PD‑L1 expression Xinling Cao1 · Guanping Zhang2 · Tao Li1 · Chengming Zhou1 · Lei Bai1 · Jinming Zhao1 · Turgunjan Tursun1 Received: 5 July 2020 / Accepted: 15 September 2020 © The Genetics Society of Korea 2020
Abstract Background Hepatocellular carcinoma (HCC) is the sixth most commonly diagnosed malignant tumor and the fourth leading cause of cancer-related deaths worldwide. As a novel non-coding RNA, LINC00657 was firstly identified as an oncogenic role in breast cancer. However, few research focus on the effect of LINC00657 on the progression of HCC. Objectives The purpose of this study was to investigate the effect of LINC00657 on HCC tissues and cells, and further explore the potential mechanism. Methods We first measured the expression of LINC00657 in HCC tissues and cell lines using qRT-PCR. Next we established LINC00657 knockdown in HCC cells. CCK-8 assay, cell invasion assay, flow cytometry analysis, qRT-PCR and western blotting were applied to assess the role of LINC00657 knockdown in the biological behavior of HCC cells. The bioinformatics analysis and the rescue experiment were devoted to the underlying mechanism. Results LINC00657 was remarkably overexpressed in HCC tissues and cell lines, associated with poor prognosis. LINC00657 knockdown repressed cell proliferation and invasion, promoted cell apoptosis of HCC cell lines. The bioinformatics analysis showed LINC00657 sponged miR-424 as a ceRNA. Besides, PD-L1 mimic rescued the suppression of si-LINC00657 in the biological behavior of HCC cells. Conclusion In a word, we observed LINC00657 regulated PD-L1 expression by sponging miR-424, thus affecting the developments of hepatocellular carcinoma. These findings LINC00657 may provide new evidence for therapeutic application in hepatocellular carcinoma. Keywords Hepatocellular carcinoma · LINC00657 · miR-424 · PD-L1
Introduction Hepatocellular carcinoma (HCC) is the sixth most commonly diagnosed malignant tumor and the fourth leading cause of cancer-related deaths worldwide (Bray et al. 2018). HCC has the characteristics of insidious onset, invasive fastgrowing, high recurrence rate and fatality (Zhu et al. 2016).
* Turgunjan Tursun [email protected] 1
Department of Liver & Laparoscopic Surgery, Digestive & Vascular Surgery Center, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Xinshi District, Ürümqi 830054, Xinjiang Uyghur Autonomous Region, China
Department of Proctology, Xinjiang General Hospital of People’s Liberation Army, Ürümqi 830000, Xinjiang Uyghur Autonomous Region, China
2
Despite remarkable improvement in diagnoses and treatments of HCC, the prognoses of patients with HCC remain unsatisfactory. Therefore, it is a key topic in cancer research to find out new therapeutic targets for HCC (Tovoli et al. 2018). Long non-coding RNA (IncRNA) is a class
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