LncRNA OIP5-AS1 upregulates snail expression by sponging miR-34a to promote ovarian carcinoma cell invasion and migratio
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(2020) 53:49 Jiang et al. Biol Res https://doi.org/10.1186/s40659-020-00315-1
Open Access
RESEARCH ARTICLE
LncRNA OIP5‑AS1 upregulates snail expression by sponging miR‑34a to promote ovarian carcinoma cell invasion and migration Xingzhi Jiang1,2* , Zhongxue Ye1, Yafen Jiang1, Wen Yu1 and Qian Fang1
Abstract Background: Although OIP5-AS1 has been characterized as an oncogenic lncRNA in many types of cancer, its role and underlying mechanism in ovarian carcinoma (OC) remains unknown. This study aimed to investigate the role of OIP5-AS1 in OC. Methods: OC tissues and non-tumor tissues (ovary tissues within 3 cm around tumors) were collected from 58 OC patients (age range 36 to 67 years old, mean age 51.4 ± 5.9 years old). The expression of OIP5-AS1 and snail in paired tissues were determined by RT-qPCR. The interaction between OIP5-AS1 and miR-34a was predicted by IntaRNA2.0 and confirmed by dual luciferase reporter assay. The effects of overexpression of OIP5-AS1 and miR-34a on the expression of snail were analyzed by RT-qPCR and Western blotting. Cell invasion and migration were analyzed by Transwell assay. Results: We observed that the expression of OIP5-AS1 and snail was upregulated and positively correlated with each other in OC. RNA–RNA interaction analysis showed that OIP5-AS1 might sponge miR-34a. In OC cells, overexpression of OIP5-AS1 resulted in the upregulated expression of snail, while overexpression of miR-34a downregulated the expression of snail. In addition, overexpression of miR-34a reduced the effects of overexpression of OIP5-AS1 on the expression of snail. In cell invasion and migration assay, overexpression of OIP5-AS1 and snail resulted in increased OC cell invasion and migration, while overexpression of miR-34a decreased OC cell invasion and migration. Moreover, overexpression of miR-34a attenuated the effects of OIP5-AS1 overexpression on OC cell invasion and migration. Conclusions: Therefore, OIP5-AS1 may upregulate snail expression in OC by sponging miR-34a to promote OC cell invasion and migration. Keywords: OIP5-AS1, OC, miR-34a, Snail Background Ovarian carcinoma (OC) is one of the most common types of malignancy in females for years [1]. In 2018, OC affected more than 22,000 new cases and caused more than 14,000 deaths in the United States [2]. OC accounts *Correspondence: [email protected] 1 Department of Gynaecology, Hwa Mei Hospital, University of Chinese Academy of Sciences, No. 41 Northeast Street, Haishu District, Ningbo 315000, Zhejiang, People’s Republic of China Full list of author information is available at the end of the article
for only 2.5% of all cancer cases in women. However, more than 5% of cancer-related deaths are caused by OC, which is mainly due to its low invasiveness and early diagnosis rate [3]. Survival of OC patients is significantly impacted by clinical stages. It has been estimated that more than 93% of OC patients at local stage can live longer than 5 years after initial diagnosis [4]. Therefore, it’s of great clinical values to develop n
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