Lipidomic analyses reveal enhanced lipolysis in planthoppers feeding on resistant host plants
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Xiaohong Zheng , Yeyun Xin , Yaxin Peng , Junhan Shan , Ning Zhang , Di Wu , 1 1 1 1 1 1 1 Jianping Guo , Jin Huang , Wei Guan , Shaojie Shi , Cong Zhou , Rongzhi Chen , Bo Du , 1 1 2 1,2 1* Lili Zhu , Fang Yang , Xiqin Fu , Longping Yuan & Guangcun He 1
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China; State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, China
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Received July 31, 2020; accepted October 10, 2020; published online November 5, 2020
The brown planthopper (BPH) (Nilaparvata lugens Stål) is a highly destructive pest that seriously damages rice (Oryza sativa L.) and causes severe yield losses. To better understand the physiological and metabolic mechanisms through which BPHs respond to resistant rice, we combined mass-spectrometry-based lipidomics with transcriptomic analysis and gene knockdown techniques to compare the lipidomes of BPHs feeding on either of the two resistant (NIL-Bph6 and NIL-Bph9) plants or a wild-type, BPH susceptible (9311) plant. Insects that were fed on resistant rice transformed triglyceride (TG) to phosphatidylcholine (PC) and digalactosyldiacylglycerol (DGDG), with these lipid classes showing significant alterations in fatty acid composition. Moreover, the insects that were fed on resistant rice were characterized by prominent expression changes in genes involved in lipid metabolism processes. Knockdown of the NlBmm gene, which encodes a lipase that regulates the mobilization of lipid reserves, significantly increased TG content and feeding performance of BPHs on resistant plants relative to dsGFP-injected BPHs. Our study provides the first detailed description of lipid changes in BPHs fed on resistant and susceptible rice genotypes. Results from BPHs fed on resistant rice plants reveal that these insects can accelerate TG mobilization to provide energy for cell proliferation, body maintenance, growth and oviposition. brown planthopper, rice plants, RNA-seq, lipidomics, lipid metabolism, RNA interference Citation:
Zheng, X., Xin, Y., Peng, Y., Shan, J., Zhang, N., Wu, D., Guo, J., Huang, J., Guan, W., Shi, S., et al. (2020). Lipidomic analyses reveal enhanced lipolysis in planthoppers feeding on resistant host plants. Sci China Life Sci 63, https://doi.org/10.1007/s11427-020-1834-9
INTRODUCTION The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a piercing-sucking insect that can damage rice both directly by ingesting sap from the phloem and indirectly by transmitting viral diseases (Sōgawa, 1982; Hibino, 1996; Hao et al., 2008; Wang et al., 2008). BPHs migrate long distances—upwards of several hundred kilometers across different countries—cause heavy yield
losses every year (Cheng et al., 2013a). BPH resistance genes have been identified and successfully integrated into rice breeding programs (Du et al., 200
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