LncRNA-AK149641 associated with airway inflammation in an OVA-induced asthma mouse model
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LncRNA-AK149641 associated with airway inflammation in an OVA-induced asthma mouse model Jie Zhang 1,2 & Yao Zhou 1 & Haiyan Gu 1 & Jiamin Zhang 1 & Heng Tang 1 & Qiangquan Rong 3 & Lina Gu 4 & Jing Pan 1,2 & Deyu Zhao 1 & Feng Liu 1 Received: 9 April 2020 / Accepted: 3 July 2020 # The Author(s) 2020
Abstract Asthma is defined as a heterogeneous disease, usually characterized by chronic airway inflammation. Long noncoding RNAs (lncRNAs) play important roles in various biological processes. To know more about the relationships between lncRNAs and asthma, gene microarray analysis was performed to screen differentially expressed lncRNAs between the lung tissue of ovalbumin (OVA) mice and control mice. Further studies showed that downregulating differentially expressed lncRNA-AK149641 by adeno-associated virus 6 (AAV6) in OVA mice inhibited airway inflammation, with improved airway compliance and resistance, diminished infiltration of inflammatory cells, as well as less secretions of mucus, tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6). Moreover, the activity of nuclear factor-kappa B (NF-κB) in the lung tissue was reduced after downregulating lncRNA-AK149641. In conclusion, we proposed that downregulation of lncRNA-AK149641 attenuated the airway inflammatory response in an OVA-induced asthma mouse model, probably in association with modulation of the NF-κB signaling pathway. Keywords lncRNA-AK149641 . OVA-induced asthma mouse model . TNF-α . IL-6 and NF-κB signaling pathway
Introduction Bronchial asthma are a heterogeneous group of respiratory diseases, which is common among children and whose incidence is increasing in developed nations (Wilson et al. 2006). It is characterized by airway inflammation and airway hyperresponsiveness (AHR) with symptoms including recurrent wheeze, cough, and shortness of breath, together with variable expiratory airflow limitation (Kim et al. 2010). It has been widely accepted that inflammatory cells, such as Jie Zhang and Yao Zhou contributed equally to this work. * Deyu Zhao [email protected] * Feng Liu [email protected] 1
Department of Respiratory Medicine, Children’s Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu, China
2
Department of Emergency Medicine, Children’s Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu, China
3
Department of Pediatrics, Gaochun People’s Hospital, Nanjing 211300, Jiangsu, China
4
Wuxi Children’s Hospital, Wuxi 214000, Jiangsu, China
eosinophils and mast cells (Doherty and Croft 2011; Galli et al. 2011; Kim et al. 2010), as well as various cytokines, including TNF-α and IL-6, take part in the airway inflammatory response of asthma (Doganci et al. 2005; Rameshwar et al. 2014; Rincon and Irvin 2012). LncRNAs are RNAs with more than 200 nucleotides without a protein-coding capability (Yang et al. 2014). Due to a lack of reading frames, lncRNAs were initially considered non-functional. However, accumulating evidence has suggested that they participate in a variety of biological and pathological processes,
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