LncRNA CTC-497E21.4 promotes the progression of gastric cancer via modulating miR-22/NET1 axis through RhoA signaling pa
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ORIGINAL ARTICLE
LncRNA CTC‑497E21.4 promotes the progression of gastric cancer via modulating miR‑22/NET1 axis through RhoA signaling pathway Wei Zong1 · Wei Feng1 · Yun Jiang1 · Yaning Cao2 · Yuchen Ke2 · Xin Shi1 · Shaoqing Ju1 · Hui Cong1 · Xudong Wang1 · Ming Cui1 · Rongrong Jing1 Received: 17 May 2019 / Accepted: 16 August 2019 © The International Gastric Cancer Association and The Japanese Gastric Cancer Association 2019
Abstract Background Long non-coding RNAs (lncRNAs) have emerged as important roles in gastric cancer (GC). However, the role of the dysregulated lncRNAs in GC remained large unknown. We investigated the clinical significance, biological function and mechanism of CTC-497E21.4 in GC. Methods Firstly, RTFQ-PCR was used to detect the expression of CTC-497E21.4 in GC. Furthermore, knockdown of CTC497E21.4 was conducted to assess the effect of CTC-497E21.4 in vitro and vivo. Subcellular localization of CTC-497E21.4 was determined by nuclear plasmolysis PCR and FISH. We also predicted CTC-497E21.4 binding miRNAs and downstream target genes and evaluated its regulation of miR-22 by acting as a ceRNA. Result CTC-497E21.4 was upregulated in GC tissues and GC cell lines (P
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