LncRNA TTN-AS1 promotes the progression of oral squamous cell carcinoma via miR-411-3p/NFAT5 axis
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Cancer Cell International Open Access
PRIMARY RESEARCH
LncRNA TTN‑AS1 promotes the progression of oral squamous cell carcinoma via miR‑411‑3p/NFAT5 axis Su‑Wei Fu* , Yan Zhang, Shen Li, Zhi‑Yan Shi, Juan Zhao and Qing‑Li He
Abstract Background: Oral squamous cell carcinoma (OSCC) is a common kind of squamous cell carcinoma of the head and neck, which is a threat to public health. Long noncoding RNAs (lncRNAs) are associated with the development of various diseases, including cancers. LncRNA titin antisense RNA 1 (TTN-AS1) is known as a crucial regulatory factor in several cancers. Nevertheless, the specific functions of TTN-AS1 in OSCC remains obscure. Methods: The expression of TTN-AS1 in OSCC samples or cells was analyzed through qRT-PCR. Colony formation assay, EdU assay, flow cytometry assay, TUNEL assay and wound healing assay were conducted to estimate the func‑ tions of TTN-AS1 in OSCC cells. RIP and luciferase reporter assays were utilized to detect the interaction between TTNAS1 and miR-411-3p as well as between miR-411-3p and NFAT5. Results: TTN-AS1 expression was stronger in OSCC cells. Knockdown of TTN-AS1 effectively restrained cell prolifera‑ tion and migration but had inductive role in apoptosis. Moreover, TTN-AS1 could function as the miR-411-3p sponge in OSCC and miR-411-3p exerted the inhibitory functions on OSCC cell growth. In addition, NFAT5 was proven as the target of miR-411-3p. Rescue assay indicated that overexpressing NFAT5 could reverse the inhibitory function of TTNAS1 depletion on cell growth. Conclusion: lncRNA TTN-AS1 contributed to the progression of OSCC via miR-411-3p/NFAT5 axis. Keywords: TTN-AS1, miR-411-3p, NFAT5, Oral squamous cell carcinoma Background Oral squamous cell carcinoma (OSCC) is one of the commonest squamous cell carcinomas occurs in the head and neck. It ranks sixth in occurrence and had a high mortality rate [1, 2]. According to many years of investigation and research, the pathogenesis of OSCC is related to the internal factors such as drinking and smoking, but its specific pathogenesis is still unclear [1, 3]. Although the surgery for OSCC is effective, the situation for the overall survival of OSCC patients is still unfavorable [4, 5]. Thus, *Correspondence: [email protected] Department of Stomatology, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, No.7 Weiwu Road, Zhengzhou 450003, Henan, China
in-depth study of the potential molecular mechanisms of OSCC is of great significance for developing new therapeutic strategies. Long noncoding RNAs (lncRNAs) are classified as the subgroup member of noncoding RNAs family with over 200 nucleotides in length, which are not able to encode proteins [6, 7]. Recently, lncRNAs are confirmed to involve in different cell progression, such as cell proliferation and cell apoptosis. Moreover, the crucial functions of lncRNAs in the occurrence and development of assorted cancers have also been reported through a flow of researches [8, 9]. Different kind of lncRNAs exerted different functio
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