Location and mapping of the NCLB resistance genes in maize by bulked segregant analysis (BSA) using whole genome re-sequ
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Location and mapping of the NCLB resistance genes in maize by bulked segregant analysis (BSA) using whole genome re-sequencing Chunlei Li & Fenglou Ling & Guihua Su & Weifeng Sun & Hongshuang Liu & Yichen Su & Xin Qi
Received: 22 May 2020 / Accepted: 2 September 2020 # Springer Nature B.V. 2020
Abstract Northern corn leaf blight (NCLB) is one of the main diseases of maize, which greatly reduces production and causes millions of dollars in losses worldwide annually. Identification of NCLB resistance genes in maize plays an important role in the crop disease resistance breeding. In this study, a BC1F1 population with 1290 individuals was constructed using high resistance line CML493 and high susceptible line PH4CV. In total, 50 resistant individuals and 50 susceptible individuals from the BC1F1 population were selected to construct 4 DNA pools (also with the two parents) which were used for high-throughput sequencing. The key candidate genes associated with NCLB resistance genes were identified using BSA-seq and KASP platforms. The results showed that 3 genes including CDPK21, HEX9, and MKKK18 were identified and this prediction was further verified by RT-qPCR. The annotation functions were sugar signaling, calcium signaling, Chunlei Li and Fenglou Ling contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01171-3) contains supplementary material, which is available to authorized users. C. Li : F. Ling : W. Sun : H. Liu : X. Qi (*) College of Agriculture, Jilin Agricultural University, Changchun 130118 Jilin, China e-mail: [email protected] C. Li : G. Su : Y. Su (*) Maize Research Institute, Jilin Academy of Agricultural Sciences/ National Engineering Laboratory for Maize, Gongzhuling 136100 Jilin, China e-mail: [email protected]
and MAPK signaling pathways. This research provided key NCLB resistance candidate genes and also provided important markers for disease resistance breeding in maize. Keywords Maize . NCLB . Disease resistance . BSASeq . KASP . Annotation function
Introduction Maize is the main food and feed crops, which plays an important role in agricultural production. Northern corn leaf blight (NCLB) was first discovered in Italy in 1876 and has become a worldwide epidemic disease, mainly in cold and humid regions. The NCLB disease also causes the infection of stalk rot and root rot pathogens, which causes the corn yield to be lost by more than 30% (Perkins and Pedersen 1987). The NCLB disease is caused by Setosphaeria turcica. It could cause infection of many plants including Zea mays, Sorghum bicolor, Sorghum sudanense, Sorghum halepense, Cynodon dactylon, and other Gramineae grass. Bergquist et al. divided the Setosphaeria turcica into two specialization types, namely sorghum specialization (Trichometa sphaeifa f sp. sorghi) and corn specialization (T. turcica f. sp. zeae) (Bergquist and Masias 1974). Similar to the disease resistance of other crops, there are two genetic types of maize resistance to NCBL disease, n
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