QTL mapping of resistance to Gibberella ear rot in maize
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QTL mapping of resistance to Gibberella ear rot in maize Jing Wen & Yanqi Shen & Yuexian Xing & Ziyu Wang & Siping Han & Shijie Li & Chunming Yang & Dongyun Hao & Yan Zhang
Received: 1 March 2020 / Accepted: 15 September 2020 # Springer Nature B.V. 2020
Abstract Gibberella ear rot (GER), caused by the fungal pathogen Fusarium graminearum, is becoming one of the most prominent pathogens responsible for ear rot in maize. In this study three F2 populations, F2-C, F2-D, and F2-J, and their corresponding F2:3 families, were constructed by crossing three highly GER-resistant inbred lines—Cheng351, Dan598, and JiV203—with the susceptible line ZW18. We used this cross for genetic analysis and QTL mapping of resistance to GER. Analysis of variance of GER in the three F2 populations revealed the presence of significant differences among genotypes and between locations. The broad-sense heritability (H2) of GER resistance was estimated to be 0.68, 0.63, and 0.64 in the three F2 populations, indicating that genetic factors play a key role in the development of phenotypic variation. Seventeen QTLs conferring resistance to GER were detected in the three F2
populations, among which the QTL qRger7.1, originating from the resistant parent Cheng351, explained 20.16–41.84% of the phenotypic variation. The physical support interval of qRger7.1 exhibited approximately 2 Mb overlap with that of qRger7.2, which was derived from the resistant parent Dan598, supporting the identification of potential “hotspots” of the target QTLs. QTLs derived from the resistant parents Dan598 and JiV203 accounted for 59.67–61.28% and 65.82– 66.90%, respectively, of the phenotypic variation. The GER-resistant QTLs identified in this study are useful candidates for improving the resistance to GER in maize using molecular marker-assisted selection. Keywords Gibberella ear rot . Maize . Resistance . QTL mapping
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01173-1) contains supplementary material, which is available to authorized users. J. Wen : Y. Shen : Z. Wang : S. Han : S. Li : C. Yang : D. Hao (*) : Y. Zhang (*) Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033 Jilin Province, China e-mail: [email protected] e-mail: [email protected] Y. Xing Maize Research Institute, Jilin Academy of Agricultural Sciences, Gongzhuling 136100 Jilin Province, China
Gibberella ear rot (GER) is a fungal disease affecting the ears of maize. It is caused by Fusarium graminearum, which also causes rot diseases in the stalks and roots of maize, and affects many other cereal crop hosts, causing related diseases (Goswami and Kistler 2004). The symptoms of GER are rot and pink mold that occur in ears from the tip side (Mesterházy et al. 2012; Brauner et al. 2017). GER reduces the yield and quality of kernels and triggers the accumulation of two major mycotoxins, deoxysqualenol and zearalenone, which cause health problems in humans and animals (Wu
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