QTL mapping for resistance to ear rot caused by Fusarium graminearum using an IBM Syn10 DH population in maize
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QTL mapping for resistance to ear rot caused by Fusarium graminearum using an IBM Syn10 DH population in maize Guangsheng Yuan & Benfang Chen & Hua Peng & Qi Zheng & Yucui Li & Kui Xiang & Li Liu & Chaoying Zou & Haijian Lin & Haiping Ding & Guangtang Pan & Zhiming Zhang
Received: 22 October 2019 / Accepted: 17 July 2020 # Springer Nature B.V. 2020
Abstract Gibberella ear rot (GER) caused by Fusarium graminearum (teleomorph Gibberella zeae), is one of the most devastating diseases in maize that not only severely reduces grain yield but also contaminates the kernels with potent mycotoxins which is harmful to livestock and humans. Identification of stable quantitative trait loci (QTL) and candidate genes conferring resistance is a promising strategy in resistance breeding programs for the disease. Here, the ten times intermated B73 × Mo17-doubled haploid (IBM Syn10 DH) population comprising a total of 298 diverse maize inbred lines were subjected to phenotypic evaluation in a 2Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01158-0) contains supplementary material, which is available to authorized users.
year field test across two environments, and the IBM Syn10 DH population were employed to detect QTL across multiple environments using an ultra-highdensity bin map. Ten QTL were detected on chromosomes 1, 2, 3, 6, 7, 8, and 9, respectively. Of them, a major QTL on chromosome 7, named qGRfg7, could explain 33.26% of the phenotypic variation. Based on fine-mapping strategy, we narrowed down the interested region into 91 kb, flanked by two developed Indel markers. A predicted gene in the mapped region, encoding a lipopolysaccharide-binding protein (LBP), is suggested to be a candidate gene conferring resistance to GER disease. These results will facilitate the breeding process for GER resistance by marker-assisted selection (MAS) in maize.
G. Yuan : B. Chen : Q. Zheng : Y. Li : K. Xiang : L. Liu : C. Zou : H. Lin : G. Pan Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China
Keywords Gibberella ear rot . Fusarium graminearum . Quantitative trait loci . Lipopolysaccharide-binding protein
G. Yuan (*) : B. Chen : Y. Li State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China e-mail: [email protected]
Introduction
H. Peng Sichuan Tourism College, Chengdu 610100 Sichuan, China H. Ding : Z. Zhang (*) State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China e-mail: [email protected]
Gibberella ear rot (GER) in maize caused by Fusarium graminearum (Schwabe) is a common disease worldwide (Reid et al. 1999; Yang et al. 2017). The spores of F. graminearum naturally infect maize upon conveyance through wind, rain splash or insect infestation, and infected kernels are seen as a reddish-pink mo
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