Lysosomes Alteration in HeLa Cell Exposed with 1-Aminocyclopropane-1-Carboxylic Acid, the Ethylene Precursor of Plant Ho
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ORIGINAL PAPER
Lysosomes Alteration in HeLa Cell Exposed with 1‑Aminocyclopropane‑1‑Carboxylic Acid, the Ethylene Precursor of Plant Hormones Mi Young Heo1 · Ra‑Mi Park1 · Bit‑Na Kim1 · Ngoc‑Han Thi Nguyen2 · Hye Weon Jang1 · Yang‑Hoon Kim3 · Jiho Min1,2 Accepted: 10 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The study of senescence preservative on cut flowers helps boost the commercial value of flowers. Senescence in cut flower is associated with an increase of ethylene production, and is significantly influenced by ethylene pathway. This study was conducted to investigate whether S-adenosyl-L-methionine (SAM) and aminocyclopropane-1-carboxylic acid (ACC) involved in the ethylene synthesis process are correlated with the lysosome. The alterations of lysosome which was treated with the ethylene precursors ACC and SAM in HeLa cell using the confocal laser scanning microscope were investigated. According to the experimental results, the activity of lysosomes increased concentration dependently by ACC treatment, however, no change was observed by SAM treatment. In addition, Liquid chromatography–mass spectrometry (LC/MS) analysis was performed to confirm the effect of lysosomal enzyme (LE) extracted from egg white on ACC reduction, but no change was observed. On the contrary, to confirm the effect of ACC on lysosomes, lysosomes were extracted from HeLa cells treated with 5 mM ACC and confirmed by FE-SEM. The results showed that the size of lysosomes treated with ACC is larger than that of the control, which was treated with distilled water. The lysosomes in the control group were distributed in various ranges from 0 to 800 nm, but those treated with 5 mM ACC were in the range of 400 nm to 800 nm or more. Therefore, lysosomes had no effect on ACC, the precursor of ethylene, the aging hormone of cut flowers, however, ACC had effect on lysosomes. Keywords Aminocyclopropane-1-carboxylic acid (ACC) · S-adenosyl-L-methionine (SAM) · Lysosome · Cut flower
Introduction The life of cut flowers plays an important role in the market. When the cut flower life is short in the distribution process, its appearance deteriorates and the marketability is lowered. Therefore, various studies have been carried out on
senescence of cut flower. One of the factors determining the vase life of cut flowers is the senescence hormone. Plant hormones such as ethylene, abscisic acid (ABA), gibberellins, auxin (IAA), and cytokinins regulate many physiological and biochemical processes in the plants [1]. In this variety of hormones, ethylene is a simple plant hormone, the simplest
* Yang‑Hoon Kim [email protected]
Hye Weon Jang [email protected]
* Jiho Min [email protected]
1
Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, 567 Baekje‑daero, deokjin‑gu, Jeonju‑si, Jeollabuk‑do 54896, Republic of Korea
2
Department of Bioprocess Engineering, Jeonbuk National University, 567 Baekje‑daero, deokjin‑gu, Jeonju‑si, Jeollabuk‑do 54896, Republic
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