MAPLE Direct Write (DW) patterning of Bio-active Ceramic Nanocomposites
- PDF / 1,008,163 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 81 Downloads / 186 Views
AA5.42.1
MAPLE Direct Write (DW) patterning of Bio-active Ceramic Nanocomposites A. Doraiswamy1, T. M. Patz1, R. J. Narayan1 L. Harris2, R. Auyeung2, R. Modi2, D.B. Chrisey2 1
Bioengineering Program & School of Material Science and Engineering, Georgia Institute of Technology, Atlanta, GA, USA 2 US Naval Research Laboratory, Washington, DC, USA
ABSTRACT A laser-based approach, Matrix Assisted Pulsed Laser Direct Write (MAPLE) technique is used to demonstrate two-dimensional direct writing of ceramics such as hydroxyl-apatite and zirconia, for developing inorganic scaffolds. We also demonstrate the patterning of live MG63 osteoblast cells onto various substrates. Our results show successful direct writing of ceramics and live cells concurrently, with a growth profile similar to that of as-deposited cells. After several days of growth, a live/dead assay shows live cells suggesting the biocompatibility of the ceramic and the viability of the process. This investigation demonstrates a novel method of developing heterogeneous tissue scaffolds, such as a cell-ceramic composite by CAD/CAM patterns.
INTRODUCTION Conventional tissue engineering approaches focus on the development of novel synthetic 3dimensional scaffold materials, and seeding cells onto the material to obtain cell proliferation and tissue function. Earlier techniques of casting and molding have given way to better techniques such as photolithography, micromachining, and various deposition methods. Matrix Assisted Pulsed Laser Evaporation- Direct Write (MAPLE-DW) is a laser based deposition technique that could be developed to generate layer-by-layer tissue scaffolds. In the past [1-3], we have successfully used the Matrix Assisted Pulsed Laser Evaporation- Direct write (MAPLEDW) approach to write mesoscopic patterns of passive electronic pastes or inks. Recently, this technique has been successfully demonstrated in depositing layers of biomaterials, bio-molecules and live-cells of various types [4-6] to develop biological scaffolds. Bio-ceramics like tricalcium phosphate and hydroxyl-apatite (HA) have been employed to develop bioactive coatings for bone-implants [7]. In the present study, we extend the MAPLE-DW layer-by-layer deposition of materials to combinations of materials such as a live-cell-ceramic (MG63 osteoblast – HA) composite to develop novel heterogeneous biological scaffolds that offer cellular-in-growth.
EXPERIMENTAL DETAILS Matrix Assisted Pulsed Laser evaporation (MAPLE) Direct write (DW) A setup as shown in Figure 1, is used for MAPLE DW. A pulsed excimer UV laser (operating at 193nm at 30ns) is used as the source light and is focused onto the ribbon plane. A 1” quartz disc is used as a ribbon with the material coated on one side. The ribbon sits on a
AA5.42.2
holder that is placed directly above the substrate. The gap between the ribbon and substrate is controlled using a z-stage translation. The x-y translation stage is controlled by CAD setup. In our experiments, the laser was operated at a constant repetition rate of 10Hz at flu
Data Loading...
