Mapping QTL for agronomic traits under two levels of salt stress in a new constructed RIL wheat population
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ORIGINAL ARTICLE
Mapping QTL for agronomic traits under two levels of salt stress in a new constructed RIL wheat population Qiaoling Luo1 · Qi Zheng1 · Pan Hu1 · Liqin Liu1 · Guotang Yang1,2 · Hongwei Li1 · Bin Li1 · Zhensheng Li1 Received: 14 November 2019 / Accepted: 16 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message QTL for 15 agronomic traits under two levels of salt stress in dry salinity field were mapped in a new constructed RIL population utilizing a Wheat55K SNP array. Furthermore, eight QTL were validated in a collected natural population. Abstract Soil salinity is one of the major abiotic stresses causing serious impact on crop growth, development and yield. As one of the three most important crops in the world, bread wheat (Triticum aestivum L.) is severely affected by salinity, too. In this study, an F 7 recombinant inbred line (RIL) population derived from a cross between high-yield wheat cultivar Zhongmai 175 and salt-tolerant cultivar Xiaoyan 60 was constructed. The adult stage performances of the RIL population and their parent lines under low and high levels of salt stress were evaluated for three consecutive growing seasons. Utilizing a Wheat55K SNP array, a high-density genetic linkage map spinning 3250.71 cM was constructed. QTL mapping showed that 90 stable QTL for 15 traits were detected, and they were distributed on all wheat chromosomes except 4D, 6B and 7D. These QTL individually explained 2.34–32.43% of the phenotypic variation with LOD values ranging from 2.68 to 47.15. It was found that four QTL clusters were located on chromosomes 2D, 3D, 4B and 6A, respectively. Notably, eight QTL from the QTL clusters were validated in a collected natural population. Among them, QPh-4B was deduced to be an allele of RhtB1. In addition, three kompetitive allele-specific PCR (KASP) markers derived from SNPs were successfully designed for three QTL clusters. This study provides an important base for salt-tolerant QTL (gene) cloning in wheat, and the markers, especially the KASP markers, will be useful for marker-assisted selection in salt-tolerant wheat breeding.
Introduction Soil salinity is one of the major abiotic stress factors which seriously constrains crop growth, development and productivity (Munns and Tester 2008; Roy et al. 2014; Wang and Xia 2017; Zhu 2002, 2016). It has been estimated that Communicated by Peter Langridge. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03689-8) contains supplementary material, which is available to authorized users. * Qi Zheng [email protected] 1
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China
University of Chinese Academy of Sciences, Beijing 100049, China
2
about 20% of irrigated and 8% of rainfed agricultural land in the world are influenced by salt (Asif et al. 2018; Munns
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