Primary mapping of QTL for growth-related traits in grass carp ( Ctenopharyngodon idellus )
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Primary mapping of QTL for growth-related traits in grass carp (Ctenopharyngodon idellus) Chengchen Yu 1,2,3 & Xiaoyan Xu 1,2,3 & Jiale Li 1,2,3 & Yubang Shen 1,2,3,4 Received: 28 December 2019 / Accepted: 4 August 2020 / Published online: 18 September 2020 # Springer Nature Switzerland AG 2020
Abstract
Growth-related traits are economically important in aquaculture, and their variation is linked to quantitative loci (QTLs). For exploring genetic basis of genetic breeding and improvement of grass carp (Ctenopharyngodon idellus), QTL mapping for growthrelated traits was performed with an F2 family of grass carp from the Yangtze River system, including 360 offspring. In this study, we mapped QTLs for body weight, body length, body height, and body width using 99 previously published microsatellite loci. A total of 15 growth-related QTLs were found in 7 linkage groups (LG1, LG2, LG14, LG15, LG16, LG18, and LG21). Four QTLs were related to body weight (qBWH1, qBWH14, qBWH15, and qBWH16), and explained 3.1 to 7.7% of the phenotypic variance. Six QTLs were related to body length (qBL1, qBL2, qBL14, qBL15, qBL16, and qBL18), and these QTLs explained 2.8 to 8.9% of the phenotypic variance. Three QTLs affecting body height (qBH14, qBH15, and qBH16) explained 3.2 to 9.2% of the phenotypic variance. Two QTLs affecting body width (qBW15 and qBW21) accounted for 9.2% and 3.3% of the phenotypic variance, respectively. In order to fine map the QTL affecting body weight on LG1 (qBWH1), 14 additional novel microsatellites were included, and the qBWH1 was relocated with a narrower flanking marker interval of 7.5 cM. This is the first study of QTL mapping in grass carp and provides new insights for the application of molecular marker–assisted breeding in this species. Keywords Ctenopharyngodon idellus . QTL . Growth . Microsatellites . Breeding
Introduction Biological traits are divided into qualitative traits and quantitative traits. Some qualitative traits are controlled by a few major genes, and the genetic relationship is relatively simple. The Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10499-02000594-1) contains supplementary material, which is available to authorized users.
* Yubang Shen [email protected] Extended author information available on the last page of the article
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Aquaculture International (2020) 28:2275–2285
quantitative traits are commonly controlled by numerous loci with small individual effects (QTLs). Most of the quantitative traits are economically important, and analyses of these traits help researchers to link certain phenotypes to specific regions on linkage groups, which is manly done by means of QTL mapping (Frankham 1996). QTL analyses have been conducted in a number of aquatic species (Yue 2014), such as growth traits (Wringe et al. 2010), meat quality traits (Derayat et al. 2007), sex determination (Alfaqih et al. 2009), and disease resistance (Ozaki et al. 2010). QTL mapping is based on the association of phenotypic data to linkage map esta
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