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Willem F. Wolkers Harriëtte Oldenhof Editors

Cryopreservation and Freeze-Drying Protocols Fourth Edition

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK

For further volumes: http://www.springer.com/series/7651

For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed.

Cryopreservation and FreezeDrying Protocols Fourth Edition

Edited by

Willem F. Wolkers Unit for Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany; Biostabilization Laboratory—Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, University of Veterinary Medicine Hannover, Hannover, Germany

Harriëtte Oldenhof Unit for Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

Editors Willem F. Wolkers Unit for Reproductive Medicine— Clinic for Horses University of Veterinary Medicine Hannover Hannover, Germany

Harrie¨tte Oldenhof Unit for Reproductive Medicine— Clinic for Horses University of Veterinary Medicine Hannover Hannover, Germany

Biostabilization Laboratory—Lower Saxony Centre for Biomedical Engineering Implant Research and Development University of Veterinary Medicine Hannover Hannover, Germany

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-0716-0782-4 ISBN 978-1-0716-0783-1 (eBook) https://doi.org/10.1007/978-1-0716-0783-1 © Springer Science+Business Media, LLC, part of Springer Nature 2015, 2021 1st edition: © Humana Press Inc., 1995 2nd edition: © Humana Press Inc., 2007 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. i