Mediation of induced systemic resistance by the plant growth-promoting rhizobacteria Bacillus pumilus S2-3-2
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ORIGINAL ARTICLE
Mediation of induced systemic resistance by the plant growthpromoting rhizobacteria Bacillus pumilus S2-3-2 Weilan Li1 · Seung‑Yeol Lee1,2 · Young‑Je Cho3 · Sa‑Youl Ghim4 · Hee‑Young Jung1,2 Received: 29 February 2020 / Accepted: 30 September 2020 © Springer Nature B.V. 2020
Abstract Plant-rhizobacteria interaction and co-evolution developed adaptive strategies which may help the plant survive in nature. Plant rhizosphere soil isolates were analyzed to investigated the effects of rhizobacteria for promoting plant growth and suppress plant disease. Bacterial strains which isolated from plant rhizosphere soil were screened for elicitation of induced systemic resistance (ISR) on tobacco. Strain S2-3-2 results in significant reduction of disease severity on tobacco, it was identified as Bacillus pumilus by multilocus sequence analysis (MLSA). Strain S2-3-2 was deeper studied for pepper plant growth promotion and biological control activity against pepper bacterial spot disease. It was found that the pepper disease severity was decreased when the roots were drenched with strain S2-3-2, and the pepper plants had a higher weight and chlorophyll content, as compared with the mock-treated plants. Transcriptional expression of pathogenesis-related (PR) protein genes in pepper was analyzed by real-time PCR, gene expressions of CaPR1, CaPR4, and CaPR10 were increased when the plants were treated with strain S2-3-2. Moreover, strain S2-3-2 was tested for the production of indole-3-acetic acid (IAA), and it was determined to emit volatiles that enhance the growth of the tobacco plants. Interesting, heat-killed S2-3-2 enhance the pepper root growth, increase the gene expressions of CaPR4 and CaPR10 after pathogen challenge for 6 h, but limited to suppress the pepper bacterial spot disease as compare to the mock-treated plants. Strain S2-3-2 can be a potential biological control agent on the plant root for plant growth promoting and disease suppression. Keywords Bacterial spot disease · Biological control · Induced systemic resistance (ISR) · Plant growth-promoting rhizobacteria (PGPR)
Introduction Rhizobacteria are closely associated with and strongly colonized on plant roots and are capable of promoting plant growth. Rhizosphere bacteria primarily refer to the beneficial rhizobacteria that have antagonistic or probiotic effects in the process of biocontrol. Rhizobacteria are also referred to as plant growth-promoting rhizobacteria (PGPR), living * Hee‑Young Jung [email protected] 1
School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
2
Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea
3
School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea
4
School of Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea
as rhizospheric or endophytic organisms on the plant root [1]. PGPR can stimulate plant growth and suppress plant diseases by producing
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