Hyper Production and Eco-Friendly Bleaching of Kraft Pulp by Xylanase From Bacillus pumilus SV-205 Using Agro Waste Mate
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ORIGINAL PAPER
Hyper Production and Eco‑Friendly Bleaching of Kraft Pulp by Xylanase From Bacillus pumilus SV‑205 Using Agro Waste Material Sushil Nagar1 · Vijay Kumar Gupta2 Received: 20 June 2020 / Accepted: 21 September 2020 © Springer Nature B.V. 2020
Abstract Hyper production of an extra cellular, alkali stable, cellulase free and thermostable xylanase have been produced from Bacillus pumilus SV-205 using wheat bran as a substrate under solid state fermentation. Enzyme production under optimized conditions enhanced the production level from 4510 to 65,130 ± 1000 IU/g dry substrate, which was 14.4 fold as compared to production under un-optimized conditions. The application of crude xylanase was investigated in pulp bio bleaching. The bio-bleaching of kraft pulp with xylanase was the most effective at an enzyme dose of 12.5 IU/g oven dried pulp, pH 10.0 and 120 min incubation at 60 °C. Under the optimized conditions, xylanase pre-treatment reduced Kappa number by 0.8 points and increased brightness by 1.08 points as compared with the control. The pre-treatment of pulp with xylanase resulted in 19.01% reduction in chlorine consumption by maintaining the same brightness as in control. These results clearly demonstrated that the B. pumilus SV-85S xylanase was effective as a pulp bio-bleaching agent. The decrease in chlorine consumption by pre-treatment of pulp with xylanase apparently made the bio-bleaching process not only economical but also eco-friendly. The xylanase from B. pumilus SV-205 exhibit remarkable properties which are suitable for application in paper pulp bleaching and an elevated production of xylanase by B. pumilus SV-205 under solid state fermentation over wheat bran, a cheap and easily available agro-residue would apparently reduce the enzyme cost substantially. Graphic Abstract
Keywords Xylanase · Xylan · Solid state fermentation · Agro-residues · Wheat bran Extended author information available on the last page of the article
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Statement of Novelty This paper reports high level of xylanase production using cost effective agro –residues under solid state fermentation conditions. Production level of xylanase Bacillus pumilus SV-205 was substantially high as per the literature available. The results are significant as these findings will be of immense use in paper and pulp industry. The bio bleaching study also revealed that enzymatic pre-treatment of pulp with cellulase free, thermo tolerant and alkali stable xylanase produced by B. pumilus SV-205 reduced the chlorine consumption by 19.01% with the same brightness level as in control. Xylanase act as an eco-friendly alternative over the conventional harsh techniques.
Waste and Biomass Valorization
process [4, 12]. The industrial level production of xylanase using the commercially available pure xylan is expensive. Hence, there is a need to increase its cost-effective production, which can be accomplished by either using an inexpensive growth substrate or selecting a micro organism producing high titre of the enzy
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