Complementary Dynamics of Banana Root Colonization by the Plant Growth-Promoting Rhizobacteria Bacillus amyloliquefacien
- PDF / 8,602,341 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 28 Downloads / 200 Views
PLANT MICROBE INTERACTIONS
Complementary Dynamics of Banana Root Colonization by the Plant Growth-Promoting Rhizobacteria Bacillus amyloliquefaciens Bs006 and Pseudomonas palleroniana Ps006 at Spatial and Temporal Scales Rocío Margarita Gamez 1
&
Sandra Ramirez 2
&
Martha Montes 3
&
Massimiliano Cardinale 4,5
Received: 1 April 2020 / Accepted: 3 August 2020 # The Author(s) 2020
Abstract Banana (Musa acuminata) growth for commercial purposes requires high amounts of chemical fertilizers, generating high costs and deleterious effects on the environment. In a previous study, we demonstrated that two plant growthpromoting rhizobacteria (PGPR), Bacillus amyloliquefaciens Bs006 and Pseudomonas palleroniana Ps006, isolated in Colombia, could partially replace chemical fertilizers for banana seedling growth. In a second work, the effects of the two inoculants on banana transcripts were found to occur at different times, earlier for Bs006 and later for Ps006. This leads to the hypothesis that the two rhizobacteria have different colonization dynamics. Accordingly, the aim of this work was to analyze the dynamics of root colonization of the two PGPR, Bs006 and Ps006, on banana growth over a time frame of 30 days. We used fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM), followed by three-dimensional reconstruction and quantitative image analysis. Bacillus amyloliquefaciens Bs006 abundantly colonized banana roots earlier (from 1 to 48 h), ectophytically on the rhizoplane, and then decreased. Pseudomonas palleroniana Ps006 was initially scarce, but after 96 h it increased dramatically and became clearly endophytic. Here we identify and discuss the potential genetic factors responsible for this complementary behavior. This information is crucial for optimizing the formulation of an effective biofertilizer for banana and its inoculation strategy. Keywords Banana cv. Williams . Plant growth-promoting rhizobacteria (PGPR) . Bacillus . Pseudomonas . Fluorescence in situ hybridization-confocal laser scanning microscopy (FISH-CLSM) . Colonization dynamics
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00248-020-01571-0) contains supplementary material, which is available to authorized users. * Massimiliano Cardinale [email protected] Rocío Margarita Gamez [email protected] Sandra Ramirez [email protected]
2
Corporación Colombiana de Investigación Agropecuaria – Agrosavia, C.I. Tibaitatá, Mosquera, Cundinamarca, Colombia
3
Corporación Colombiana de Investigación Agropecuaria – Agrosavia, C.I. Caribia, Zona Bananera, Magdalena, Colombia
4
Institute of Applied Microbiology, Justus-Liebig-University Giessen, Giessen, Germany
5
Department of Biological and Environmental Sciences and Technologies – DiSTeBA, University of Salento, Centro Ecotekne via Provinciale Lecce-Monteroni, I-73100 Lecce, Italy
Martha Montes [email protected] 1
Corporación Colombiana de Investigación Agropecuaria – Agrosavia, C.I. Tur
Data Loading...
