Membrane Protein Structure and Function Characterization Methods and
In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowled
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Jean-Jacques Lacapere Editor
Membrane Protein Structure and Function Characterization Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Membrane Protein Structure and Function Characterization Methods and Protocols
Edited by
Jean-Jacques Lacapere Sorbonne Universités—UPMC Univ Paris 06 École Normale Supérieure—PSL Research University Département de Chimie, CNRS UMR 7203 LBM, Paris, France
Editor Jean-Jacques Lacapere Sorbonne Universite´s—UPMC Univ Paris 06 , E´cole Normale Supe´rieure—PSL Research University De´partement de Chimie, CNRS UMR 7203 LBM Paris, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7149-7 ISBN 978-1-4939-7151-0 (eBook) DOI 10.1007/978-1-4939-7151-0 Library of Congress Control Number: 2017943682 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Membrane proteins are essential components of biological processes such as ions, metabolites or water transport, signal transduction, sensing cell environment, and control of cell-cell contact. Dysfunction of these proteins induces numerous human pathologies like cystic fibrosis which originates from a genetic disorder that is linked to the most common case to the deletion of one amino acid. Many other disorders like cancers and inflammatory diseases occur through cell-cell communications and involve membrane proteins. Although many studies have been performed to characterize mem
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